tailieunhanh - Isolation, cloning and sequencing of Phlacbd gene cluster encoding antibiotic and Phloroglucinol derivative 2, 4-Diacetylphloroglucinol (2, 4- DAPG) from Pseudomonas Fluorescens

Pseudomonas represents as one of the most abundant class of bacterial genus in many antagonistic root-associated communities. Pseudomonas spp. residing in the rhizosphere has the ability to produce antimicrobial metabolites including phloroglucinol derivatives 2,4-DAPG, which is a major class of secondary metabolite protecting plants against different types of phytopathogens. The present study was conducted to isolate 2,4-DAPG producing Pseudomonas fluorescens isolates from Rhizosphere soil samples of different crops. Among a total of 158 isolates screened for the production of 2,4-DAPG through PCR based approach, seven isolates were found to be positive for DAPG. Further, the antibacterial activities of these Pseudomonas isolates were evaluated in vitro against Xanthomonas axonopodis pv punicae and it was found that the isolate AFPF19 exhibited highest antagonistic activity against phytopathogen. AFPF19 isolate was used to clone DAPG synthesizing gene cluster phlACBD into cloning vector pTZ257R/T and confirmed by sequencing. | Isolation, cloning and sequencing of Phlacbd gene cluster encoding antibiotic and Phloroglucinol derivative 2, 4-Diacetylphloroglucinol (2, 4- DAPG) from Pseudomonas Fluorescens