tailieunhanh - Bioremediation of crude oil polluted terrestrial soil using Aspergillus clavatus and Pichia spp.

In Nigeria, crude oil pollution challenge of the terrestrial environment is very prevalent. Soil fungi play very important role in the degradation of organic materials, as well as agents of the biogeochemical cycles. Fungi have an advantage over bacteria due to their production of hyphae that can penetrate contaminated soil. The aim of this study was to evaluate the bioremediation potential of two fungal species: Aspergillus clavatus and Pichia spp. Crude oil contaminated soil samples were collected from Numuu Mitee, Kegbara-Dere community in Gokana Local Government Area of Rivers State; Nigeria. There were four experimental setups for the bioremediation study; Soil without organisms served as control(A), soil with Aspergillus clavatus alone (B), soil sample with Pichia spp. alone (C), while soil with Aspergillus clavatus and Pichia spp combined (D). Standard microbiological methods were used to analyze total heterotrophic and hydrocarbon utilizing fungi. The following physicochemical parameters; pH, nitrate, phosphate, sulphate, and total hydrocarbon content (THC) were analysed for baseline and monitored every 7days for 28days. Molecular identification of the organisms was also carried out using 16S rRNA amplification. The results of the baseline were as follows; pH , nitrate 52mg/kg, phosphorus 149mg/kg, THC 8,, total heterotrophic fungi x 104 cfu/g and hydrocarbon utilizing fungi x 103 cfu/g. | Bioremediation of crude oil polluted terrestrial soil using Aspergillus clavatus and Pichia spp.

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