tailieunhanh - Genetic response of sugarcane (Saccharum officinarum L.) genotypes to varying concentrations of Cytokinins for in vitro shoot multiplication

Studies were carried out for rapid micropropagation of two sugarcane genotypes Co-86032 and CoVC-18061. The explants were surface sterilized with 75% alcohol for 30 minutes using cotton. The cultures were initiated by inoculating shoot apical meristem on MS (Murashige and Skoog, 1962) medium containing mg/l kinetin. The multiplication response of two sugarcane genotypes was studied under five levels of 6-Benzylaminopurine (0, , 1, and 2 mg/l) and five levels of kinetin (0, , , and mg/l) in completely randomized design with 5x5x2 factorial treatment combinations. Analysis of variance (ANOVA) showed that the interaction effects of 6-benzlyaminopurine (6-BAP), kinetin and the sugarcane genotypes on number of shoots per explant, shoot length, and chlorophyll content was highly significant (p | Genetic response of sugarcane (Saccharum officinarum L.) genotypes to varying concentrations of Cytokinins for in vitro shoot multiplication

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