tailieunhanh - In vitro sterilization protocol for establishment of litchi (Litchi chinensis Sonn) cv. Purbi

Litchi is considered a crop difficult to propagate through micropropagation and obtaining contamination free cultures is first and foremost requirement of tissue culture. So the present investigation was carried out to standardize the sterilization procedure of nodal segment and leaf explants of Litchi chinensis Sonn. cv. Purbi. Two surface sterilizing agents viz. HgCl2 and NaOCl were used at varying concentrations and durations. | In vitro sterilization protocol for establishment of litchi (Litchi chinensis Sonn) cv. Purbi (2020) 9(3): 831-835 International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 9 Number 3 (2020) Journal homepage: Original Research Article In vitro Sterilization Protocol for Establishment of Litchi (Litchi chinensis Sonn) cv. Purbi Neha Nischal, Hidayatullah Mir, Shaheena Parveen*, Shashi Prakash, Ruby Rani and Sanjay Sahay Department of Horticulture (Fruit & Fruit Technology), Bihar Agricultural University, Bhagalpur, 813210, Bihar, India *Corresponding author ABSTRACT Litchi is considered a crop difficult to propagate through micropropagation and obtaining contamination free cultures is first and foremost requirement of tissue culture. So the present investigation was carried out to standardize Keywords the sterilization procedure of nodal segment and leaf explants of Litchi Litchi, chinensis Sonn. cv. Purbi. Two surface sterilizing agents viz. HgCl2 and Micropropagation, NaOCl were used at varying concentrations and durations. In this NaOCl, HgCl2, Mortality experiment HgCl2 was found to be better sterilizing agent than NaOCl for Article Info both the explants. HgCl2 ( %) for 3 min treatment was found to be the most effective for nodal segment explants that resulted in maximum per Accepted: cent survival (±), low percent mortality (±) and low 05 February 2020 Available Online: per cent contamination (±). While for leaf explants HgCl2 ( 10 March 2020 %) for min yielded best results with highest per cent survival (±), low per cent mortality (±) and low per cent contamination (±). Introduction Sapindaceae or soapberry family and sub family Nepheleae which comprises not less Litchi (Litchi chinensis Sonn.) .