tailieunhanh - Diagnosis of bovine Sarcocystosis by conventional methods

Sarcocystis species are considered as one of the most prevalent parasites of bovines. Sarcocysts occur either macroscopically or microscopically in the tissues of bovine intermediate hosts. Sarcocystosis being an occult infection, most of the time diagnosis in intermediate host by clinical symptoms is unreliable. In this study, the macrosarcocysts were observed in the infected muscle sample by naked eye examination. Suspected muscle samples were examined by muscle squash technique which showed the presence of microsarcocysts in the muscle tissue and bradyzoites were recovered through muscle digestion technique. The detailed morphology of the recovered bradyzoites was studied by Giemsa, Leishman’s and Diff Quick staining which revealed banana shaped bradyzoites with a clear nucleus towards the posterior end. The trypan blue staining indicated live (colourless) and dead (blue) bradyzoites and the gliding movement of the bradyzoites was recorded. | 2019 8 2 941-947 EXCELLENT PUBLISHERS International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 8 Number 02 2019 Journal homepage http Original Research Article https Diagnosis of Bovine Sarcocystosis by Conventional Methods Rasmita Panda Manchukonda Udaya Kumar . Sreenivasa Murthy and . Reddy Department of Veterinary Parasitology College of Veterinary Science PVNR TVU Rajendranagar Hyderabad-500 030 Telangana India Corresponding author ABSTRACT Keywords Bovine Sarcocystis Bradyzoites Staining Article Info Accepted 10 January 2019 Available Online 10 February 2019 Sarcocystis species are considered as one of the most prevalent parasites of bovines. Sarcocysts occur either macroscopically or microscopically in the tissues of bovine intermediate hosts. Sarcocystosis being an occult infection most of the time diagnosis in intermediate host by clinical symptoms is unreliable. In this study the macrosarcocysts were observed in the infected muscle sample by naked eye examination. Suspected muscle samples were examined by muscle squash technique which showed the presence of microsarcocysts in the muscle tissue and bradyzoites were recovered through muscle digestion technique. The detailed morphology of the recovered bradyzoites was studied by Giemsa Leishman s and Diff Quick staining which revealed banana shaped bradyzoites with a clear nucleus towards the posterior end. The trypan blue staining indicated live colourless and dead blue bradyzoites and the gliding movement of the bradyzoites was recorded. Introduction Sarcocystosis is characterised by cyst formation in muscle tissue muscular sarcocyctosis in the intermediate host or colonization of lamina propria of the intestines intestinal Sarcocystosis in definitive host. Sarcocystis has a requisite two-host life cycle based on a prey-predator intermediate-definitive host relationship Fayer 2004 . The .