tailieunhanh - Molecular detection and characterization of Niger [Guizotia abyssinica (L.f.) Cass] phyllody phytoplasma

Niger [Guizotia abyssinica (.) Cass.] is one of the important minor oilseed crops in India. Phyllody disease on niger noticed for the first time at Main Research Station, Hebbal, Bengaluru, Karnataka. The molecular detection and characterization of the phytoplasma causing niger phyllody was investigated during Kharif 2016. The causal agent of the phyllody disease was identified based on symptoms, amplification of 16S rDNA of the phytoplasma by polymerase chain reaction (PCR) from infected samples, as well as by sequencing and phylogenetic analysis. The molecular detection by using nested PCR phytoplasma specific universal primers R16F2n/R16FR2 revealed the amplification of phytoplasmal specific PCR product of kb fragment corresponding to the 16S rDNA. The16S rDNA sequence of niger phyllody phytoplasma had maximum nucleotide identity of 90 per cent with the 16S rDNA sequence of Cymbopogon citratus white leaf phytoplasma (), Alfalfa witches'-broom Phytoplasma Mes 38(), Sesamum indicum phyllody Phytoplasma () and Vigna radiata phyllody NDL (). | Molecular detection and characterization of Niger [Guizotia abyssinica (.) Cass] phyllody phytoplasma