tailieunhanh - Characterization and molecular modelling of Pi56 Ortholog from Oryza Rufipogon

Rice genome harbours many resistance genes (R-genes) with tremendous allelic diversity, constituting a robust immune system effective against microbial pathogens like rice blast fungus M. oryzae. Nevertheless, few functional R-genes have been identified for rice blast resistance. Wild species of cultivated plants are treasure trove for important agronomic traits. The wild rice Oryza rufipogon is resistant to many virulent strains of Magnaporthe oryzae. Although considerable research on characterizing genes involved in biotic stress resistance is accomplished at genomic and transcript level, characterization at proteins level is yet to be explored. In the present study, we report the amplification, sequencing and protein sequence analysis of Pi56ortholog (Pi56or) in O. rufipogon accession WRA21. The Pi56or encodes 746 amino acid protein with an isoelectric point of analysis revealed that Pi56or shared highest similarity (80%) with Oryza meridionalis ortholog. The predicted 3D model confirmed 17 α helices and 18β pleated sheets with ATP-binding site close to β sheet present towards the N-terminus of the protein molecule. The present study using various molecular and bio-computational tools could, therefore, help in improving our understanding of this key resistance protein and prove to be a potential target towards developing resistance to M. oryzae in rice. | Characterization and molecular modelling of Pi56 Ortholog from Oryza Rufipogon

• Sinh học
• Oryza rufipogon
• Rice blast
• NBSLRR domain
• Pi56 Ortholog
• Molecular modelling
• BMC Chemistry
• Homology modelling
• Molecular dynamics
• Molecular docking
• Antifungal activities
• Inhibition mechanism
• Cajanus cajan
• Functional domain
• Acyrthosiphon pisum
• Cajanus cajan lectin against aminopeptidase n receptor
• BMC Developmental Biology
• Mathematical modelling
• Planar polarity
• Self organisation
• In silico phenotyping
• Thiazole derivatives
• Antimicrobial activity
• Anticancer activity
• BMC Bioinformatics
• Kinase inhibitors
• Ligand charge state
• ATP mimic
• Molecular dynamics simulation
• Collision detection
• 2D hand drawings
• Multiple collaborators
• BMC Molecular and Cell Biology
• Human papilloma virus
• HPV E6
• Apoptotic pathway
• Polymer Stabilizers
• Molecular Implications
• Probability
• Catalyst Composition
• Cancer Therapy
• Calculus of Variations
• Solution Techniques
• Integral Transforms
• Complex Variables
• scientific reports
• medical reports
• health education
• medical knowledge
• medical research
• analysis of cytoplasmic
• Cellular Automata
• Logical Computation
• Quantum Computations
• Automata Application
• Organizing Networks
• Molecular Orbitals
• Angiotensin converting
• combination therapies
• Health Action International
• Saliva Testing
• Molecular Epidemiology
• HAART and Causes
• Modelling
• Virtual Crack Closure
• Technique and Finite
• Predicting the Delamination
• Growth Initiation
• Composite Structures
• Report medicine
• traditional medicine
• oxidation
• Crystal structure
• bacteria
• breast cancer
• medicine
• chemical reaction
• Parasitic Diseases
• Northwest Mexico
• Bacterial Diseases
• Molecular Detection
• Characterization
• Virulence Associated
• genetics
• Structural biochemistry
• Structural Biology
• Chalcone synthase
• Cell suspension culture
• P coumaroyl CoA
• Chromatography A
• Beer marker
• Human urine
• Molecularly imprinted polymers
• International reference
• Development Goal
• Median price
• Plant Antioxidative
• Heavy Metal Toxicity
• Genetic and Molecular