tailieunhanh - Detection of Vancomycin resistant Enterococci in various clinical sample isolates from a tertiary care centre

Enterococci are Gram-positive, facultative anaerobic organisms, ovoid in shape, short chains, in pairs or single cells. The common enterococci-associated nosocomial infections are urinary tract infections, surgical wound infections and bacteremia. Resistance to vancomycin is now widely reported worldwide. In India, the prevalence of VRE has been reported as’ 8%, and 23% in New Delhi, Chandigarh, and Mumbai. This study was done to detect the presence of vancomycin resistant enterococci (VRE) among the various samples in a tertiary care hospital. A total of 100 non duplicate isolates of Enterococci were collected from different clinical samples. Antibiotic susceptibility testing was done by Kirby Bauer disc diffusion method. Vancomycin resistance was detected using Vancomycin screen agar and E strip method. Out of 100 Enterococcal isolates,66 isolates were found to be multi drug resistant 4 isolates (4%) were presumptively identified as vancomycin resistant by vancomycin screen agar containing 6 μg/ml vancomycin and E strip method. All the 4 VRE isolates were resistant to Penicillin and Ciprofloxacin. 50% percent of the isolates were sensitive for High level Gentamycin and Teicoplanin. 100% percent of the isolates were sensitive to Linezolid and Quinipristin. Enterococci are emerging as an important pathogen causing variety of nosocomial infections and also cause community acquired infections contributing significantly to patients morbidity and mortality. The emergence of Vancomycin resistant Enterococci worsens the problem further because of the Multidrug resistance exhibited by these agents leaving fewer therapeutic options for the clinicians in treating the serious life threatening VRE infections. | Detection of Vancomycin resistant Enterococci in various clinical sample isolates from a tertiary care centre