tailieunhanh - Identification of candidate gene based markers (SNPs) in the ZIP family transporter sequences of rice (Oryza sativa L.)

Iron and Zinc are the key ingredient in human nutrition, soil and crop management. Rice is the major dietary source for the population in India but it is deficient in iron and zinc. An effort was made to quantify the iron and zinc content in brown, white and cooked white rice and to study the effect of nucleotide variations in genes associated with zinc. 14 genotypes were selected and grown in field at UAS, GKVK in RCBD, during Kharif-2014. Quality parameter zinc in brown rice showed negative effect on yield. Highly positively significant correlation was observed for grain yield with number of productive tillers. Literature survey revealed genes belonging to transporter family OsZIP are involved in transporting zinc. Primers were designed specific to these genes. The primer profile at gel level was monomorphic for all the primers. Sequencing the amplicons of primersOsZIP2-1 and OsZIP7-2 revealed that variations were present in all these amplicons but nucleotide changes at ninth position of OsZIP2-1 were significantly associated with brown rice zinc content and nucleotide changes at 25, 40 and 551 positions of OsZIP7-2 were significantly associated with grain iron content. Although OsZIP2-1 and OsZIP7-2 were monomorphic at gel level nucleotide variations were significantly associated with grain iron and zinc content. The markers identified in this study can be used for improving the grain iron and zinc in rice. | Identification of candidate gene based markers (SNPs) in the ZIP family transporter sequences of rice (Oryza sativa L.)