tailieunhanh - Effects of calcium chloride treatment on suppression of fruit anthracnose disease caused by colletotrichum gloeosporioides

Bananas, chilies and oranges are the most widely grown plants in Viet Nam. However, their fruits are severely affected by anthracnose disease caused by Colletotrichum gloeosporioides. Therefore, the objective of this study was to evaluate the effects of CaCl2 on invitro mycelial growth and in vivo lesion diameters on pisang awak bananas, oranges and chillies . | Effects of calcium chloride treatment on suppression of fruit anthracnose disease caused by colletotrichum gloeosporioides AGU International Journal of Sciences – 2019, Vol 7 (3), 75 – 83 EFFECTS OF CALCIUM CHLORIDE TREATMENT ON SUPPRESSION OF FRUIT ANTHRACNOSE DISEASE CAUSED BY COLLETOTRICHUM GLOEOSPORIOIDES Le Thanh Toan1, Vo Thi Huong Duong2, Nguyen Thi My Linh1, Vo Trong Ky1, Trieu Phuong Linh1 1 Can Tho University 2 An Giang University, VNU - HCM Information: ABSTRACT Received: 25/02/2019 Bananas, chilies and oranges are the most widely grown plants in Viet Accepted: 09/10/2019 Nam. However, their fruits are severely affected by anthracnose disease Published: 11/2019 caused by Colletotrichum gloeosporioides. Therefore, the objective of this Keywords: study was to evaluate the effects of CaCl2 on invitro mycelial growth and in Anthracnose, CaCl2, chili, vivo lesion diameters on pisang awak bananas, oranges and chillies . orange, pisang awak banana. Solution of CaCl2 at three concentrations of 20, 40 or 60 mM was mixed into medium of PDA at temperature of approximately 55-60ºC, gently shaken and poured into petri dish with a volume of approximately 10 mL per one petri. A hyphal round slice of Colletotrichum was put at the center of each petri dish. Diameter of colony was recorded at 2, 4 and 6 days after putting the fungal slice. Then, fully matured fruits were inoculated with a spore suspension of C. gloeosporioides at a density of 106 spore mL-1, treated with CaCl2 20mM by soaking the fruits for 30 seconds. The results showed that the solution of CaCl2 at concentrations of 20, 40 and 60 mM highly inhibited hyphal development of Colletotrichum at in vitro conditions. Moreover, the calcium chloride solution at 20 mM protected the fruits from anthracnose damage. 1. INTRODUCTION important and destructive disease in bananas, Fruits including bananas, oranges and chillies oranges and chillies. On unripe