tailieunhanh - In vitro micropropagation of the orchid (dendrobium crystallinum var. alba)

The results showed that protocorms were formed from seeds culture on the ½ MS medium supplemented with 10% potatoes extract within 8 weeks. Protocorms which were cultured on the ½ MS medium containing 30 g/l sucrose, g/l AC, 7 g/l agar, mg/l NAA and 1 mg/l BA were optimal for PLB formation ( PLBs/sample) after 8 weeks culture. Protocorms converted into normal plants with well-developed shoots and roots on the ½ MS medium supplemented with 20 g/l sucrose, 10% potatoes extract, g/l AC, and 7 g/l agar after about 90 days. | In vitro micropropagation of the orchid (dendrobium crystallinum var. alba) AGU International Journal of Sciences – 2019, Vol 7 (3), 1 – 8 IN VITRO MICROPROPAGATION OF THE ORCHID (DENDROBIUM CRYSTALLINUM VAR. ALBA) Tran Thi Ngoc Lan1 1 South Central and Highland Institute of Science Information: ABSTRACT Received: 07/08/2018 Studies of micropropagation of Dendrobium crystallinum var. alba. were Accepted: 17/10/2018 conducted in order to conserve and develop this precious orchid species. The Published: 11/2019 results showed that protocorms were formed from seeds culture on the ½ MS Keywords: medium supplemented with 10% potatoes extract within 8 weeks. Protocorms BA, Dendrobium crystallinum which were cultured on the ½ MS medium containing 30 g/l sucrose, g/l AC, var. alba., NAA, PLB, 7 g/l agar, mg/l NAA and 1 mg/l BA were optimal for PLB formation ( protocorm. PLBs/sample) after 8 weeks culture. Protocorms converted into normal plants with well-developed shoots and roots on the ½ MS medium supplemented with 20 g/l sucrose, 10% potatoes extract, g/l AC, and 7 g/l agar after about 90 days. PLBs converted into normal plants on the same medium supplemented with mg/l NAA and mg/l BA, too. No abnormal morphological changes were found in these Dendrobium seedlings. 1. INTRODUCTION Micropropagation provided an important method for mass propagation of many orchid species. Vietnam has a tropical monsoon climate that is There were perusals of Dendrobium appropriate for orchid growth such as the micropropagation on the world and Vietnam. Dendrobiums. Dendrobiums are not a beautiful Sunitibala & Rajkumar (2009) cultured seeds of species although they are highly valued in the Dendrobium transparens L. on the ½ Murashige flower industry as potted plants and cut flowers. and Skoog (½ MS) suplemented with 1 mg/l NAA Even though these wild orchids are diverse, they and 2 mg/l BA. Nguyễn Văn