tailieunhanh - Genome sequence analysis and identification of genes associated to pesticide degradation from Enterobacter Cloacae strain MR2

Today's burning problem in the world is pesticide residues in foods. To overcome this problem, nineteen chlorpyrifos-degrading bacteria were isolated from soil of adjoining area of pesticide manufacturing industries located in Gujarat, India. The strain CPD-12 (MR2) degraded highest chlorpyrifos among the other strains isolated from different sites, . Up to 500 ppm in 30 hrs. And hence was selected further for whole genome sequencing. This strain showed maximum similarity to members of the order Enterobacteriales and was closest to Enterobacter cloacae of this group. The genome sequence of strain Enterobacter cloacae MR2 consisted of a circular 4,758,062bp chromosome with a G +C value, 5571 protein coding genes, 16rRNA and 72 tRNAs. The genome annotation and functional characterization of the strain MR2 provided insights into various genetic processes involved in the degradation of several pesticides and detoxification of toxic compounds. The genome of MR2 was also compared with Enterobacter cloacae subsp cloacae ATCC 13047 and Enterobacter sp. 638 which showed the presence of genes for the pesticide degradation as in ATCC 13047 and also had genes to promote plant growth as in Enterobacter sp. 638. | Genome sequence analysis and identification of genes associated to pesticide degradation from Enterobacter Cloacae strain MR2

• Sinh học
• Whole genome sequencing
• Chlorpyrifos degradation
• Enterobacter cloacae MR2
• Draft genome
• Enterobacter Cloacae strain MR2
• BMC Genetics
• Whole exome sequencing
• Next generation sequencing
• Complex diseases
• Inflammatory bowel diseases
• Variant priorization
• Variants of unknown significance
• BMC Bioinformatics
• Genome coverage
• Sequencing depth
• Multisample calling algorithms
• BMC Genomics
• HLA genotyping
• Cancer immunogenomics
• Bayesian hierarchical model
• Markov chain Monte Carlo
• High coverage whole genome sequencing
• Variant analysis
• Vietnamese human genome
• Illumina sequencing machines
• Borrelia miyamotoi
• Reference genome
• Long read sequencing
• Lyme borreliosis
• Genome Biology
• Genome wide association studies
• Mapping precision
• False positive rate
• Protein coding genes
• Exome sequencing
• BMC Veterinary Research
• Tiling PCR
• Amplicon sequencing
• Nanopore sequencing
• Structural variation
• Copy number variation
• Human diseases
• Pan genome
• Accessory genome
• BMC Cancer
• Precision oncology
• Whole genome amplification
• Single cell sequencing
• Multiple displacement amplification
• Variant calling
• Human genomes
• Genome sizing
• Genome analysis
• Whole genome
• Nextgeneration sequencing
• DNA sequence
• Batch effects
• Principal components analysis
• De novo genome assembly
• Molecular epidemiology
• Strain typing
• Antimicrobial resistance
• Molecular typing
• Differentially methylated region
• DNA methylation
• Whole genome bisulfite sequencing
• Disease susceptibility
• Long read whole genome sequencing
• Dynamic time warping
• Variable number tandem repeat
• ATP binding cassette
• HPV integration
• High risk human papillomaviruses
• Mycobacterium tuberculosis
• Drug resistant tuberculosis
• Within patient diversity
• Human microbiome
• Microbiome composition
• Helicobacter pylori
• Data structure
• Whole genome alignment
• Next generation sequencing technologies
• Genome wide association study
• Fine mapping
• Longread sequencing
• Type I interferon genes
• Cell free DNA
• Colorectal cancer
• Early stage cancer
• Analysis pipeline
• Analysis workflow
• Reproducible workflows
• Short tandem repeats
• DNA forensics
• Eukaryotic genomes