tailieunhanh - Detection of circulating dengue virus serotypes in a tertiary care centre in central Kerala, 2016

Dengue is the most common mosquito-borne viral infection in tropical and sub-tropical countries. Dengue virus, a single stranded positive sense RNA virus belongs to the genus Flavivirus in the family Flaviviridae. Both Aedes aegypti and Aedes albopictus are the main vectors for dengue virus in India. Dengue illnesses are caused by one or more of the serologically related viruses namely DENV-1, DENV-2, DENV-3, DENV-4 and the newly identified has faced a dramatically expanded dengue virus transmission over the last few decades, with rapidly changing epidemiology. In recent years, India has reported increased incidences of concurrent infection with multiple serotypes of dengue viruses (DENV). In the present study, we have identified the circulating dengue virus serotypes in a tertiary care centre in Central Kerala in 2016. A prospective study was conducted in the Department of Microbiology, of a tertiary care centre in Central Kerala from January 2016 to December 2016. A total of 274 adult patients whose serum samples were NS1 positive were further subjected to conventional multiplex RT- PCR. Out of 274, Dengue RT PCR was positive for 159 (58%) samples. Of 159, 64() were identified as DENV-1, 55 () DENV-2, seven () DENV-3, two () were DENV-4. Twenty- two cases () were co-infected with DENV- 1 and DENV-2, six () with DENV-2 and DENV-4 and three () with DENV-2 and DENV-3. All the four dengue serotypes circulated in Kerala in the year 2016, of which DENV-1 was found to be the predominant serotype followed by DENV-2. | Detection of circulating dengue virus serotypes in a tertiary care centre in central Kerala, 2016