tailieunhanh - Egg mass Mycoflora of Meloidogyne incognita in Assam, India

Survey was conducted during 2014-15 for the isolation of mycoflora from the egg masses of Meloidogyne incognita infecting crops like tomato, brinjal, pea and ameranthus from five different locations viz., Charigaon, Alengmora, Danichopari, Namdeori and Barbheta of Jorhat and Golaghat district of Assam. The egg masses were collected and surface sterilized in per cent sodium hypochlorite (NaOCl) for two minutes. Further these egg masses were washed thoroughly with sterile distilled water until the traces of NaOCl was removed and placed in potato dextrose agar plate. The inoculated pertriplates were incubated at 25±2oC in BOD incubator for 4 days. A pure culture of each isolate was made by using hyphal tip technique. A total of 29 fungal isolates comprising of 7 genera with 9 species viz. Trichoderma harzianum, Paecilomyces niphetodes, Acremonium falciforme, Fusarium oxysporium, F. solani, Aspergillus niger, A. flavus, Vermispora leguminacea, Penicillium spp. and an unidentified species were recovered. All the species showed varied relative frequency of occurrence, F. oxysporum being the most frequently occurred species with per cent of total fungal isolates. | 2019 8 1 1616-1634 EXCELLENT PUBLISHERS International Journal of Current Microbiology and Applied Sciences ISSN 2319-7706 Volume 8 Number 01 2019 Journal homepage http Original Research Article https Egg Mass Mycoflora of Meloidogyne incognita in Assam India Kurulkar Uday1 B. Bhagawati1 . Neog3 Dutta Pranab2 and M. Annapurna1 1 Department of Nematology 2Department of Plant Pathology Departmem of Nematology . Assam Agricultural University Jorhat Assam India Corresponding author ABSTRACT Keywords Eggmass Meloidogyne incognita T. harzianum P. niphetodes A. falciforme F. oxysporium F. solani A. niger A. flavus V. leguminacea Penicillium Jorhat and Golaghat Article Info Accepted 12 December 2018 Available Online 10 January 2019 Survey was conducted during 2014-15 for the isolation of mycoflora from the egg masses of Meloidogyne incognita infecting crops like tomato brinjal pea and ameranthus from five different locations viz. Charigaon Alengmora Danichopari Namdeori and Barbheta of Jorhat and Golaghat district of Assam. The egg masses were collected and surface sterilized in per cent sodium hypochlorite NaOCl for two minutes. Further these egg masses were washed thoroughly with sterile distilled water until the traces of NaOCl was removed and placed in potato dextrose agar plate. The inoculated pertriplates were incubated at 25 2oC in BOD incubator for 4 days. A pure culture of each isolate was made by using hyphal tip technique. A total of 29 fungal isolates comprising of 7 genera with 9 species viz. Trichoderma harzianum Paecilomyces niphetodes Acremonium falciforme Fusarium oxysporium F. solani Aspergillus niger A. flavus Vermispora leguminacea Penicillium spp. and an unidentified species were recovered. All the species showed varied relative frequency of occurrence F. oxysporum being the most frequently occurred species with per cent of total fungal isolates. .