tailieunhanh - Regeneration of haploids in oil seed through embryonic microspore

Anther Culture or microspore culture is a very important and useful tool in plant breeding for efficient production of haploids and subsequent doubled haploid plants in many species, limited research has been conducted with Brassica –rapaas it is more recalcitrant in cell and tissue culture than other Brassicas. In order to access the effect of genotype on microspore embryogenesis in B –rapa, two high yielding brown Sarsoon cultivars (KS-101 and KOS-1) were studied for their response to haploid plant regeneration through Anther culture. Flower buds of mm to mm size representing late uninucleate stage of anther microspores were subjected to low temperature treatment 40C for 7days and anthers from these buds were cultured on MS medium having 13% sucrose. Anthers were then sub cultured in MS media supplemented with various concentrations of auxins and cytokinins. The best callus development and proliferation was achieved in MS media supplemented with 2,4-D 1mg/l and mg/l NAA. | Nội dung Text Regeneration of haploids in oil seed through embryonic microspore

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