tailieunhanh - Effect of extraction conditions on the antioxidant activity of Vernonia amygdalina Del. (Asteraceae)

In this study, the effect of extraction conditions on the antioxidant activity of Vernonia amygdalina Del. (Asteraceae) was evaluated by Response surface methodology and central composite design (RSM-CCD) to predict the content of phenolic compounds with maximum antioxidant activity. | Effect of extraction conditions on the antioxidant activity of Vernonia amygdalina Del. (Asteraceae) TẠP CHÍ PHÁT TRIỂN KHOA HỌC VÀ CÔNG NGHỆ - 37 KỸ THUẬT & CÔNG NGHỆ, TẬP 1, SỐ 3, 2018 Effect of extraction conditions on the antioxidant activity of Vernonia amygdalina Del. (Asteraceae) Dinh Chung Duong*, Ngoc Yen Nguyen Thi, Hung Lam Hoa are evidences that the regression model can Abstract—In this study, the effect of represent the experimental data well. HPLC extraction conditions on the antioxidant activity showed that leaves contain at least six of Vernonia amygdalina Del. (Asteraceae) was flavonoids, two of which are apigenin and evaluated by Response surface methodology luteolin. The flavonoids apigenin and luteolin and central composite design (RSM-CCD) to were identified in the extract from Vernonia predict the content of phenolic compounds with amygdalina with high levels of apigenin ( maximum antioxidant activity. Total phenol mg/g dw), luteolin ( mg/g dw). and flavonoid contents were determined by Keywords—Vernonia amygdalina Del., spectrophotometry method, especially the extraction conditions, polyphenol, antioxidant flavonoid content was identified by HPLC-DAD activity, oxidative stress. system. The antioxidant activity was estimated by the DPPH and the FRAP method. Results 1 INTRODUCTION showed that extracting time, extracting temperature and solvent-to-material ratio had a significant effect on phenolic content (p < F ree radicals play important roles and necessary for life. It was produced continuously in all cells as part of a normal cellular function. Free ). The interactions between the three radicals and oxidants contain both toxic and factors were also found to be significant at beneficial compounds. Oxidative stress, arising as level of probability. After re-estimating a result of an imbalance between free radical predicted variables on the experiment, we production