tailieunhanh - Roles of dehydrin genes in wheat tolerance to drought stress

Physiological parameters and expression levels of drought related genes were analyzed in early vegetative stage of two bread wheat cultivars (Sids and Gmiza) differ in drought tolerance capacity. Both cultivars were imposed to gradual water depletion started on day 17 till day 32 after sowing. Sids, the more tolerant cultivar to drought showed higher fresh and dry weights than the drought sensitive genotype, Gmiza. Under water stress, Sids had higher membrane stability index (MSI), lower accumulated H2O2 and higher activity of the antioxidant enzymes; catalase (CAT), guaiacol peroxidase (GPX), ascorbate peroxidase (APX) and superoxide dismutase (SOD) than Gmiza. On the other hand, the differential expression patterns of the genes dhn, wcor and dreb were observed due to water deficit intensity according to cultivar’s tolerance to drought. The DNA sequence alignment of dun showed high similarity of about 80–92% identities with other related plants. The most striking overall observed trend was the highly induction in the expression of dun, wcor and dreb in leaves of the tolerant genotype, Sids under severe water stress. | Journal of Advanced Research 2015 6 179-188 Cairo University Journal of Advanced Research ORIGINAL ARTICLE Roles of dehydrin genes in wheat tolerance to drought stress CrossMark Nemat M. Hassan Zeinab M. El-Bastawisy Ahamed K. El-Sayed Heba T. Ebeed Mamdouh M. Nemat Alla Botany Department Faculty of Science Damietta University Egypt ARTICLE INFO ABSTRACT Article history Received 9 June 2013 Received in revised form 14 November 2013 Accepted 15 November 2013 Available online 21 November 2013 Keywords Antioxidants dhn dreb Drought stress wcor Wheat cultivars Physiological parameters and expression levels of drought related genes were analyzed in early vegetative stage of two bread wheat cultivars Sids and Gmiza differ in drought tolerance capacity. Both cultivars were imposed to gradual water depletion started on day 17 till day 32 after sowing. Sids the more tolerant cultivar to drought showed higher fresh and dry weights than the drought sensitive genotype Gmiza. Under water stress Sids had higher membrane stability index MSI lower accumulated H2O2 and higher activity of the antioxidant enzymes catalase CAT guaiacol peroxidase GPX ascorbate peroxidase APX and superoxide dismutase SOD than Gmiza. On the other hand the differential expression patterns of the genes dhn wcor and dreb were observed due to water deficit intensity according to cultivar s tolerance to drought. The DNA sequence alignment of dun showed high similarity of about 80-92 identities with other related plants. The most striking overall observed trend was the highly induction in the expression of dun wcor and dreb in leaves of the tolerant genotype Sids under severe water stress. 2013 Production and hosting by Elsevier . on behalf of Cairo University. Introduction Drought is a wide world problem constraining global crop production seriously 1 . Common wheat possesses a very complex and huge genome with size of I6 X 109 bp which consists of about 90 repeated sequences 2 . Under drought stress H2O2

• Sinh học
• Drought stress
• Wheat cultivars
• Roles of dehydrin genes
• Tolerance to drought stress
• Drought stress
• PEG induced drought stress
• PEG induced drought stress on seed germination
• Seedling characters of maize
• Seedling characters
• Crop plants
• Drought avoidance
• Drought tolerance
• Tolerance mechanisms
• Post anthesis drought
• Drought susceptibility index
• Biochemical responses of soybean
• Post anthesis drought stress
• Biochemical responses
• Plant Biology
• Differentially expressed genes
• Drought adaptation model
• Drought tolerant mutant
• Transcriptomic analysis
• Zea mays
• Effect of nitrogen application
• Dry matter production
• Nitrogen application levels on growth of rice
• Growth of rice under drought stress conditions
• Drought stress conditions
• Identification and sequence analysis
• Sequence analysis of a DREB subfamily transcription
• DREB subfamily transcription factor involved
• Drought stress tolerance from rice
• Drought stress tolerance
• DNA Methylation
• DNA analysis
• Drought stress in maize
• Differential DNA methylation
• BMC Plant Biology
• Leaf senescence
• Hordeum vulgare L
• Premature leaf senescence
• Bt cotton
• Physiological responses
• Bt cotton under drought stress
• Physiological responses of Bt cotton
• Seed germination
• Maize genotypes
• Agricultural science
• Generation mean analysis in wheat
• Gene action
• Scaling test
• Physiological response
• Early growth stage
• Physiological responses of sugarcane
• Sugarcane to drought stress
• BMC Genomics
• Drought adaptive gene
• Drought responsive gene
• Pterocarya stenoptera
• Relative yield
• Indian mustard
• Drought stress response of Indian mustard
• Indian mustard (Brassica juncea L
• Triticum aestivum
• TaGAPCp promoter
• Drought resistance
• Morphological characterization
• Polyethylene glycol
• Reduced production
• Fine roots
• Gossypium hirsutum L
• Mechanisms underlying
• Soil drought remains unclear
• Pearl millet
• Pacbio sequencing
• Illumina sequencing
• Heat stress
• Glycine max
• water stress
• P5CS genes
• PAP3 genes
• BZIP genes
• Helianthus annuus
• Antioxidant enzymes
• Stress determining parameters
• Polyethylene glycol mediated
• ACC deaminase
• Abiotic stress
• Groundnut (Arachis hypogaea L
• Fluorescent pseudomonad isolates
• Characterization of efficient drought stress tolerant
• Rice GRAS
• Transcription factor
• Stress responsive genes
• Physic nut
• Gene expression profiles
• Abscisic acid
• Waxes and fatty acids
• Endoplasmic reticulum stress response
• Brassica juncea
• High temperature stress
• Differential gene expression
• Transcription factors