tailieunhanh - Nghiên cứu tác dụng ức chế Enzym Acetylcholinesterase và quét gốc tự do DPPH của cây Bơ (Persea Americana Mill.)

Dược liệu là một nguồn tiềm năng chứa các chất có khả năng ức chế enzym Acetylcholinesrerase (AChE) –enzym đích quan trọng trong điều trị bệnh Alzheimer. Trong nghiên cứu này, chúng tôi đánh giá tác dụng ức chế AChE và tác dụng chống oxy hóa của cây Bơ (Persea Americana Mill) gồm lá, quả, hạt và vỏ quả. Các mẫu được chiết siêu âm bằng ethanol 50% và tiến hành phân đoạn lần lượt với n-hexane, ethyl acetate (EtOAc) và n-butanol (n-BuOH). | Nghiên cứu tác dụng ức chế Enzym Acetylcholinesterase và quét gốc tự do DPPH của cây Bơ (Persea Americana Mill.) VNU Journal of Science: Medical and Pharmaceutical Sciences, Vol. 35, No. 1 (2019) 19-30 Original Article Evaluating the Acetylcholinesterase Inhibitory and Antioxidant Activities of Persea Americana Extracts Dang Kim Thu*, Hoang Thi Thuy, Bui Thi Thanh Duyen, Luc Thi Thanh Hang, Nguyen Thi Trang, Bui Son Nhat, Tran Thi Quynh Hoa, Duong Thi Ky Duyen, Bui Thanh Tung VNU School of Medicine and Pharmacy, 144 Xuan Thuy, Cau Giay, Hanoi, Vietnam Received 13 May 2019 Revised 16 May 2019; Accepted 21 June 2019 Abstract: Medicinal plants are a potential source of enzyme acetylcholinesrerase (AChE) inhibitors, a key target in the treatment of Alzheimer’s disease. This paper studies the AChE inhibitory activity and the antioxidant effect of Persea Americana Mill extract. The sample leave, seed, exocarp and mesocarp of avocado were extracted with 50% ethanol and subsequently fractionated with n-hexane, ethyl acetate (EtOA) and n-butanol (n-BuOH) solvents. The AChE inhibitory activity was evaluated by Ellman’s colorimetric method and the antioxidant activity by screening DPPH free radicals. The results show that the seed of Persea Americana extract had the strongest AChE inhibitory activity and antioxidant effect, followed by the leave extract, and the exocarp extract and mesocarp extract were the weakest. The Persea Americana seed extract inhibited AChE activity in a dose-dependent manner with an IC50 value of ± μg/mL and the antioxidant effect with an IC50 value of ± µg/mL. The results also show that n–BuOH fraction of Persea Americana seed extract had strong AChE inhibitory and antioxidant activities with an IC50 value of ± µg/ml and ± μg/mL, respectively. The study results suggest that the Persea Americana Mill is a promising ingredient in Alzheimer’s