tailieunhanh - Article 3: Characterization of the stress-induced gene ZmCOI6.1 in maize: Expression and Promoter sequences
Using cDNA subtraction technique, 18 cold stress responsive-genes were identified, among them a novel gene, , whose function is still unknown. Analysis of the promoter sequence revealed several conserved stress-responsive cis-acting elements. Further expression characterization shows that is induced, in addition by cold, by other abiotic stresses such as drought and NaCl as well as by signalling molecules such as ABA and SA. The results indicate that is a general stress responsive gene. A possible regulation mechanism is presented where is alternatively spliced yielding two transcripts whose levels are changed upon different stress treatments. Furthermore the predicted amino acid sequence and its homologue show high similarity with proteins in rice and Arabidopsis suggesting that it belongs to a conserved protein in plants. | Article 3: Characterization of the stress-induced gene in maize: Expression and Promoter sequences
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