tailieunhanh - Phân tích số liệu Microarray của rễ cây lúa bị nhiễm Asen bằng phần mềm chuyên dụng Easygo và Mapman
Bài viết tiến hành phân tích những thay đổi hệ phiên mã của tế bào rễ cây lúa sau 24 giờ xử lý với As bằng kỹ thuật microarray. Kết quả cho thấy một số lượng lớn các gen thay đổi mức độ biểu hiện (720 gen). | Phân tích số liệu Microarray của rễ cây lúa bị nhiễm Asen bằng phần mềm chuyên dụng Easygo và Mapman VNU Journal of Science: Natural Sciences and Technology, Vol. 35, No. 3 (2019) 15-21 Original Article Microarray Data Analysis of as-induced Rice Roots by using Easygo and Mapman Softwares Nguyen Thi Thuy Quynh1, , Pham Le Ngoc Han2, Vo Khanh Tam2, Phung Thi Kim Hue2, Tsai-Lien Huang 1 VNU University of Education, 144 Xuan Thuy, Cau Giay, Hanoi, Vietnam 2 Hung Vuong gifted high school, 48 Hung Vuong, Pleiku, Gia Lai, Vietnam 3 Departement Life Sciences, National Cheng kung University, Taiwan, ROC Received 22 January 2019 Revised 17 March 2019; Accepted 26 July 2019 Abstract: Rice is one of the most important crops in Asian countries such as China, Vietnam. Many recent reports indicate that the arsenic content in rice exceeds the threshold and affects human health. Studying of molecular mechanisms and finding the arsenic resistance genes in rice which is extremely important and urgent. In this study, we analyzed the transcriptional changes of arsenic- treated rice root cells during 24 hours by microarray technique. Results showed that a large number of the differentially expressed genes (720 genes). EasyGO and Mapman softwares are powerful tools in analyzing microarray data and classifying functional groups as well as the important metabolic pathways in the cell. Results of microarray analysis using EasyGO showed that 74 down-regulated genes related to cellular component, 200 up-regulated genes involved in catalytic activity, 93 up- regulated genes involved in biological processes as responding to environmental stress, and 64 detoxification-realted genes are increased expression such as cytochrome P450, Glutathione-S- transferase and UDP-Glycosyltransferase. Mapman's microarray analysis reaults also indicate that numerous of arsenic-tolerance genes of rice roots. These results support for .
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