tailieunhanh - Nghiên cứu khả năng ứng dụng dịch chiết củ Gừng (Zingiber officinale Rosc) để chế tạo hạt nano bạc và đánh giá khả năng kháng khuẩn

Tổng hợp nano bạc bằng phương pháp sử dụng các dịch chiết thực vật đang được nhiều nhóm nghiên cứu trên thế giới chú trọng vì những ưu điểm như chất lượng đồng nhất, tận dụng được nguồn nguyên liệu sẵn có và thân thiện môi trường. | Nghiên cứu khả năng ứng dụng dịch chiết củ Gừng (Zingiber officinale Rosc) để chế tạo hạt nano bạc và đánh giá khả năng kháng khuẩn VNU Journal of Science: Natural Sciences and Technology, Vol. 35, No. 3 (2019) 118-127 Original Article The Ability of Ginger Rhizome (Zingiber officinale Rosc) Extract in Producing of Silver Nanoparticles and the Antibacterial activity of these nanoparticles Ho Thi Phuong, Nguyen Thi Le Na, Nguyen Trung Thanh, Nguyen Dinh Thang Faculty of Biology, VNU University of Science, 334 Nguyen Trai, Hanoi, Vietnam Received 02 July 2019 Revised 24 July 2019; Accepted 25 July 2019 Abstract: Recently, using plant extract as a reducing agent for nanosilver particle synthesis has been focused. This is a green technology utilizing the ready material in the nature to create the nanoparticles with good properties and uniqe quality. In this study, ginger rhizome extract was used to reduce the silver cation (Ag+) to silver (Ago) as nanoparticles with uniqe quality and even distribution in the solution. The size of the particles varied in the range of 20-40 nm. Reaction conditions were investigated and optimized with AgNO3 concentration of 3mM, extract solution/AgNO3 solution of 1/5, temperature of 80˚C, pH of 12 and reaction time of 30 min. The results obtained from the antibacterial assays showed that silver nanoparticle solution had antibacterial ability with an average effective diameter of 10 mm. It also indicated that the antibacterial activity of silver nanoparticle solution on the Gram (-) bacterium (E. coli) is better that on Gram (+) bacterium (S. aureus). In conclusion, we suggest that the ginger rhizome extract can be used to produce silver nanoparticles in mild reaction conditions; the silver nanoparticle solution expressed as a quite good antibacterial agent and therefore could be applied in decreasing the effects of deleterious bacteria. Keywords: Silver nanoparticle, .