tailieunhanh - Biểu hiện gen CYP2E1 ở người lao động trong cơ sở sản xuất sơn có tiếp xúc nghề nghiệp với dung môi hữu cơ
Bài viết phân tích mức độ biểu hiện của gen CYP2E1 ở 118 người, trong đó 73 người làm việc tại công ty sản xuất sơn có tiếp xúc với BTEX và 45 người không tiếp xúc với BTEX thông qua phương pháp Real time – PCR sử dụng chất phát quang là SYBR Green. | Biểu hiện gen CYP2E1 ở người lao động trong cơ sở sản xuất sơn có tiếp xúc nghề nghiệp với dung môi hữu cơ VNU Journal of Science: Natural Sciences and Technology, Vol. 35, No. 3 (2019) 71-79 Original Article Expression of CYP2E1 gene in paint workers occupationally exposed to organic solvents Nguyen Thi Hien1,2, Do Thi Cam Nhung2, Nguyen Phu Hung3, Bui Phuong Thuan1, Nguyen Quang Huy1, 1Faculty of Biology, VNU University of Science, 334 Nguyen Trai, Hanoi, Vietnam 2Vietnam National Insitute of Occupational Safety and Health, 99 Tran Quoc Toan, Hanoi, Vietnam 3Thai Nguyen University of Sciences, Tan Thinh, Thai Nguyen, Vietnam Received 07 May 2019 Revised 16 May 2019; Accepted 12 July 2019 Abstract: Benzene (B), toluene (T), ethylbenzene (E) and xylene (X) are used commonly in paint industry, so workers who are a high risk of exposure to organic solvent (VOCs). CYP2E1 gene encodes CYP2E1 which plays an important role in the metabolism and bio-activation of volatile organic compounds. When workers expose to VOCs, their body will have a mechanism to metabolize the toxic. The enhancement of mRNA expression of CYP2E1 is a very sensitive and accurate biological marker, which is the basis for the next study to propose the level of mRNA expression of CYP2E1 gene as a human biological indicator to monitor to workers occupationally exposed to VOCs. In this study, we studied the level of mRNA expression of CYP2E1 gene from 118 participants that including 73 workers of exposed group from the paint factories and 45 workers of non-exposed group from garment factories by using Realtime-PCR with SYBR Green – an asymmetrical cyanine dye used as a nucleic acid stain in molecular biology. The initial results, showed that the exposed group had a higher mRNA expression level of CYP2E1 than the non- exposed group approximately times, and this difference was statistically significant (P value < ). And
đang nạp các trang xem trước
