tailieunhanh - Efficient conjugation of aflatoxin B1 with bovine serum albumin for the development of aflatoxin B1 quick test

Among several methods to detect the toxic and cancerous aflatoxins in agricultural products used in foods and animal feeds, only the lateral flow immunodipstick method is suitable for field usage. The aim of this study is to determine optimal conditions for the conjugation of aflatoxin B1 (AFB1) with bovine serum albumin (BSA) to develop a lateral flow immunoassay test strip for detection of AFB1. | Vietnam Journal of Science and Technology 56 (4A) (2018) 190-198 EFFICIENT CONJUGATION OF AFLATOXIN B1 WITH BOVINE SERUM ALBUMIN FOR THE DEVELOPMENT OF AFLATOXIN B1 QUICK TEST Truong Quoc Phong1, *, Pham Thi Ngoc2, Nguyen Dieu Huong1, Nguyen Thi Ngoc Anh1 1 School of Biotechnology and Food Technology, Hanoi University of Science and Technology, Dai Co Viet, Hai Ba Trung, Ha Noi 2 National Institute of Veterinary Research, Truong Chinh, Ha Noi * Email: Recieved: 23 July 2018; Accepted for publication: 6 October 2018 ABSTRACT Among several methods to detect the toxic and cancerous aflatoxins in agricultural products used in foods and animal feeds, only the lateral flow immunodipstick method is suitable for field usage. The aim of this study is to determine optimal conditions for the conjugation of aflatoxin B1 (AFB1) with bovine serum albumin (BSA) to develop a lateral flow immunoassay test strip for detection of AFB1. Optimal conditions for the generation of the intermediate compound AFB1-CMO were: AFB1/CMO ratio of 1:2, AFB1 concentration of 8 mM, reflux temperature of 80 oC, reaction time of 1 hour. The optimal conditions for further conjugation of AFB1-CMO with bovine serum albumin (BSA) were: AFB1-CMO/BSA ratio of 40:1 in bicarbonate buffer pH , reaction temperature of 25 oC, reaction time of 2 hours. The generated conjugate AFB1-BSA was used to construct a lateral flow immunoassay test strip for the detection of aflatoxin B1. Keywords: aflatoxin B1, aflatoxin B1- BSA conjugate, quick test. 1. INTRODUCTION Aflatoxins are carcinogen secondary metabolites produced mainly by the fungi Aspergillus flavus and Aspergillus parasiticus [1]. These fungi contaminate a wide range of agricultural products, such as peanuts, corn, rice, and animal feed [2]. Due to appropriate climate for fungi growth, the rate of aflatoxin contaminated agricultural products and food in Vietnam is very high ( %). Twenty three to sixty seven .

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