tailieunhanh - Role of antibiotics in chilled storage of sperm in grass carp (Ctenopharyngodon idella)

The objective of the present study was to evaluate the effect of antibiotics on chilled storage sperm motility of grass carp (Ctenopharyngodon idella). The extenders were used in this study were HBSS (Hanks’ balanced salt solution), Modified HBSS, CCSE-2 (common carp sperm extender), Kurokuda-1 and Kurokuda-2. | Journal of Fisheries science and Technology No. 4 - 2018 ROLE OF ANTIBIOTICS IN CHILLED STORAGE OF SPERM IN GRASS CARP (Ctenopharyngodon idella) Le Minh Hoang¹, Dinh Van Khuong¹ Received: ; Revised: ; Accepted: ABSTRACT: The objective of the present study was to evaluate the effect of antibiotics on chilled storage sperm motility of grass carp (Ctenopharyngodon idella). The extenders were used in this study were HBSS (Hanks’ balanced salt solution), Modified HBSS, CCSE-2 (common carp sperm extender), Kurokuda-1 and Kurokuda-2. The dilution ratios were 1:1, 1:3 and 1:5 (sperm:extender). Two antibiotics Cephalexin and Amoxcelin were used in this study at the concentration of 50, 100 or 150 ppm. The experiments were conducted in a refrigerator at the temperature of 4ºC. The results showed that the sperm motility was the highest and activated to day 9 when Kurokuda-2 was used as the extender at the dilution ratio of 1:3. The sperm motility can be maintained until day 13 by adding 25ppm Cephalexin combined with 25ppm Amoxceline to extender. Keywork: Grass carp, Ctenopharyngodon idella, sperm, chilled storage, extender, antibiotic. I. INTRODUCTION Chilled storage of fish sperm is a useful biotechnique that facilitates hatchery operations. It reduces the need of frequent collection of sperm from males, enables transportation of sperm to distant locations and prevents problems related to asynchrony in gamete production between males and females. Sperm chilled storage is affected by extenders, dilution ratio, temperature, and antibiotics (Le et al. 2011; Le et al. 2014). However, the presence of microorganisms in chilled samples may decrease fertilization and lower cell and viability (Segovia et al. 2000). To address this issue,, antibiotics are commonly added to chilled storage of sperm, but the effect of antibiotics on the chilled sperm storage of the grass carp, an important freshwater species in aquaculture, has not been tested. Grass

• Ngư nghiệp
• Grass carp
• Ctenopharyngodon idella
• Chilled storage
• Common carp sperm extender
• Hanks balanced solution
• Nutritional quality
• Sensory quality
• Thermal processing
• Smoking and thermal processing
• BMC Genomics
• Linkage map
• Growth related trait
• Quantitative trait loci
• Single nucleotide polymorphism
• Population structure
• Genetic diversity
• Genome wide population surveys
• Freshwater foodfish species
• High stocking density
• Immune function
• Grass carp transcriptome
• kỹ thuật nuôi cá
• đặc điểm của cá
• các loài cá nước ngọt
• dinh dưởng thủy sản
• tài liệu về nuôi cá
• scientific reports
• Report medicine
• traditional medicine
• molecular Biology
• tumor cells
• Antimicrobial
• Cá trắm cỏ
• đặc tính Cá trắm cỏ
• họ cá vảy
• giống cá có vảy
• kỹ thuật nuôi cá có vảy
• phân loại cá có vảy
• Bệnh học thủy sản
• Bệnh thường gặp ở thủy sản
• Cá trắm cỏm
• Bệnh ở cá trắm cỏ
• Dấu hiệu bệnh ở cá trắm cỏ
• Bệnh thận ở cá trắm cỏ
• Specialized agronomic
• environmental resources
• aquaculture
• economic
• social
• scientific research
• BMC Bioinformatics
• Hemorrhagic disease
• Protein protein interactions
• Grass carp reovirus
• Non structural proteins
• BMC Genetics
• Ctenopharyngodon idellus
• Cyprinus carpio haematopterus
• Microsatellite DNA
• DNA fragment