tailieunhanh - The spindle of oocyte observed by polarized light microscope can predict embryo quality

Objectives: To evaluate spindle position of mataphase (M) II oocyte and the development of embryos origined from oocytes with spindle and without spindle. Subjects and methods: 250 MII oocytes were analyzed with polarized microscopy in In vitro Fertilization Centre of Vietnam Military Medical University. | Journal of military pharmaco-medicine no3-2018 THE SPINDLE OF OOCYTE OBSERVED BY POLARIZED LIGHT MICROSCOPE CAN PREDICT EMBRYO QUALITY Nguyen Thanh Tung*; Le Thanh Huyen* SUMMARY Objectives: To evaluate spindle position of mataphase (M) II oocyte and the development of embryos origined from oocytes with spindle and without spindle. Subjects and methods: 250 MII oocytes were analyzed with polarized microscopy in In vitro Fertilization Centre of Vietnam Military Medical University. Results: Spindles were detected in 170 () of 218 MII oocytes, 115 spindles () of MII oocytes was beneath or adjacent to the first polar body, 55 oocytes 0 0 had the spindle located between 30 and 180 angle away from the first polar body. Fertilization rate and good quality embryos in oocytes with a visible spindle ( and ) were higher than in oocytes without a visible spindle ( and ), the difference was significant with p Bad (14/118) (3/19) > Good embryos rate of MII oocytes with visible spindle was higher than of MII oocytes with invisible spindle ( and ). The difference was statistically significant with p < . DISCUSSION 1. The presence of spindle on mature oocytes. In this research, oocytes were observed at 39 hours after hCG administration, the time that Cohen et al (2004) had observed the spindle of MII oocytes with the highest rate () [2]. In 218 observed MII oocytes, there were 170 visible spindle oocytes (), similar to the result of Cohen et al (2004), Heindryclx et al (2011) [2, 4] (585/770 oocytes (76%); 891/1089 oocytes (), respectively). In this research, in spite of 48 oocytes with the first polar body, the rate of invisible spindle oocytes was . To explain the absence of spindle in MII oocytes, Montag et al (2006) used time-lapse to observe the oocyte maturation in the in-vitro and showed that when MI oocytes extruded the first polar body, a spindle had established the .