tailieunhanh - Studies on the effect of maleic hydrazide on root tip cells and pollen fertility in Trigonella foenum graecum L.

The abnormalities noticed were both physiological and clastogenic. Fragments, stickiness of chromosomes, univalents, multivalents, and anaphase bridges were the most frequent abnormalities. The decrease in seed germination and pollen fertility was found significant at level. | Turk J Bot 32 (2008) 337-344 © TÜBİTAK Research Article Studies on the Effect of Maleic Hydrazide on Root Tip Cells and Pollen Fertility in Trigonella foenum-graecum L. Farha JABEE, Mohammad Yunus K. ANSARI, Danish SHAHAB* Cytogenetics and Mutation Breeding Lab, Department of Botany, Aligarh Muslim University, Aligarh-202002 . INDIA Received: Accepted: Abstract: In the present investigation, the effect of maleic hydrazide (MH) has been studied on seed germination, mitosis, and pollen fertility of Trigonella foenum-graecum L. to test its cytotoxicity. Six different concentrations (, , , , and ) of maleic hydrazide (MH) were given at seed level. The abnormalities noticed were both physiological and clastogenic. Fragments, stickiness of chromosomes, univalents, multivalents, and anaphase bridges were the most frequent abnormalities. The decrease in seed germination and pollen fertility was found significant at level. Mitotic index reduced gradually as the concentrations of Maleic Hydrazide increased and it was found significant at and levels. Key Words: Cytotoxicity, Fenugreek, Maleic Hydrazide (MH), Mitotic Index (MI) Introduction Fenugreek (Trigonella foenum-graecum L.), an annual legume cash crop of India (Fazli & Hardman, 1978), commonly known as “methi”, is cosmopolitan in distribution, believed to be native to the Mediterranean region, and now widely cultivated in most parts of the world (Acharya et al., 2006). It can also be efficiently incorporated into short-term crop rotations in western Canada (Moyer et al., 2003). Fenugreek has been reported to generate a high yield of good quality forage and hay or silage (Mir et al., 1998). In addition, the small inconspicuous flowers from fenugreek had a 4% success rate when crossed (Choudhury & Singh, 2001), making hybridization a less attractive route for crop improvement. Mutation breeding has become increasingly popular in recent times as .