tailieunhanh - Determination of phytogenetic relationships among isoetes species using random primers

Isoetes L. is an ancient, primitive genus of lycopsids. The genus occupies a unique position in plant evolution as the closest relatives of tree lycopods. The morphological characters, namely leaf size, comb, and sporophyll size, are used diagnostically to help in the identification of the Isoetes species. | Turk J Bot 31 (2007) 367-372 © TÜB‹TAK Research Article Determination of Phytogenetic Relationships among Isoetes Species Using Random Primers Yogendra K. TRIPATHI1, Priyatansh GURHA2, D. GHOSH3, . KUMAR4, Ved PRAKASH4 1 Department of Genetics, Barkatullah University, Bhopal - INDIA 2 Department of Molecular Biology, University of Illinois, Illinois - USA 3 Department of Genetics and Plant Breeding, Calcutta University, Kolkata - INDIA 4 National Research Center for Agroforestry, Jhansi - INDIA Received: Accepted: Abstract: Isoetes L. is an ancient, primitive genus of lycopsids. The genus occupies a unique position in plant evolution as the closest relatives of tree lycopods. The morphological characters, namely leaf size, comb, and sporophyll size, are used diagnostically to help in the identification of the Isoetes species. Thus, species identification of Isoetes is always problematic and the knowledge on the genetic relationship of Isoetes species is also limited. Therefore, in order to find a suitable method for species identification and estimation of genetic relationship among different species of Isoetes, a Random Amplified Polymorphic DNA (RAPD) technique based on Polymerase Chain Reaction (PCR) was used for the described purpose. Out of 150 decamer primers tested, 58 produced good amplification products. A total of 4442 amplification products were scored and only 3479 bands () were found to be polymorphic, with sizes ranging from 200 to 5500 bp. Unweighted pair group method using arithmetic average cluster analysis revealed a clear genetic difference among Isoetes species. The scientific data presented in this study suggest that RAPD-PCR could be a valuable tool for identification and estimation of genetic relationship among species of Isoetes L. Key Words: Isoetes species, RAPD fingerprint, identification, genetic relationship Introduction Isoetes is a non-flowering lycopod inhabiting submerged and wet areas. .