tailieunhanh - Construction of a genetic linkage map of citrus with random amplified polymorphic DNA (RAPD) markers using a progeny population from a complex intergeneric cross

A genetic linkage map of the progeny population was constructed with these RAPD markers. The map contains 63 markers distributed into 9 linkage groups, which possibly correspond to the 9 haploid chromosomes of Citrus. The total maximum length of the linkage map generated in this study was cM, with an average map distance of cM between markers. | Turk J Bot 31 (2007) 79-86 © TÜB‹TAK Research Article Construction of a Genetic Linkage Map of Citrus with Random Amplified Polymorphic DNA (RAPD) Markers Using a Progeny Population from a Complex Intergeneric Cross 1, 2 Mehtap fiAH‹N ÇEV‹K *, Gloria A. MOORE 1 Department of Horticultural Sciences, Faculty of Agriculture, Süleyman Demirel University, 32260 Isparta - TURKEY 2 Department of Horticultural Sciences, Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL, 32611, USA Received: Accepted: Abstract: This study included 30 plants that were randomly selected from a larger progeny population obtained from a complex intergeneric cross of {C. grandis (L.) Osb. x [C. paradisi Macf. x Poncirus trifoliata (L.) Raf.]} x {[(C. paradisi Macf. x P. trifoliata (L.) Raf.) x C. reticulata Blanco] x [(C. paradisi Macf. x Poncirus trifoliata (L.) Raf.) x C. sinensis (L.) Osb.]}. Genomic DNA was extracted from leaf samples of these plants and analysed for polymorphisms by polymerase chain reaction (PCR), using 10-mer random primers. A total of 111 random amplified polymorphic DNA (RAPD) markers were identified using 38 random primers. A genetic linkage map of the progeny population was constructed with these RAPD markers. The map contains 63 markers distributed into 9 linkage groups, which possibly correspond to the 9 haploid chromosomes of Citrus. The total maximum length of the linkage map generated in this study was cM, with an average map distance of cM between markers. Key Words: Citrus, DNA markers, RAPD markers, genetic linkage mapping Rastgele Ço¤alt›lm›fl DNA (RAPD) Markörleri ve Cinsler Aras› Karmafl›k Melez Populasyonu Kullan›larak Turunçgil Genetik Haritas›n›n Oluflturulmas› Özet: Cinsler aras› kamafl›k {C. grandis (L.) Osb. x [C. paradisi Macf. x Poncirus trifoliata (L.) Raf.]} x {[(C. paradisi Macf. x P. trifoliata (L.) Raf.) x C. reticulata Blanco] x [(C. paradisi Macf. x Poncirus trifoliata .