tailieunhanh - In vitro and in vivo germination of Cyclamen alpinum seeds

Cyclamen trochopteranthum has a confusing history, having been identified in the last part of the 19th century and described as Cyclamen alpinum Sprenger. It remained known as C. alpinum until 1975, when it was described as C. trochopteranthum by Otto Schwarz. Cyclamen trochopteranthum is a species distributed in the southwestern part of Turkey. | Research Article Turk J Bot 33 (2009) 277-283 © TÜBİTAK doi: In vitro and in vivo germination of Cyclamen alpinum seeds Betül BÜRÜN1,*, Oral ŞAHİN2 1 Muğla University, Faculty of Science and Arts, Department of Biology, Muğla - TURKEY 2Muğla University, Ortaca Vocational School, Muğla - TURKEY Received: Accepted: Abstract: Cyclamen trochopteranthum has a confusing history, having been identified in the last part of the 19th century and described as Cyclamen alpinum Sprenger. It remained known as C. alpinum until 1975, when it was described as C. trochopteranthum by Otto Schwarz. Cyclamen trochopteranthum is a species distributed in the southwestern part of Turkey, but the C. trochopteranthum described in 1975 is today describe again as Cyclamen alpinum. The seeds of this species were collected from red pine forests in the Gökbel-Dalyan area on May 3-7 and stored with and without capsules in incubators at 20 °C. Among the seeds that were sown 5-6 months later (October-November), the germination rate was 35% at 15 °C and 47% at 20 °C in October. Germination increased to 80% at 15 °C and 87% at 20 °C in November. The highest germination rate was observed in December (95%) and these seeds had been stored for 8 months. Seeds that were stored for 8 months (using different storage methods) were subjected to in vitro and in vivo germination tests at 15 °C and 20 °C. Seed storage method (in capsules and out of capsules) did not have a significant effect on in vitro germination in Murashige-Skoog medium, but storing seeds in capsules had a significant effect on germination on filter paper and in vivo (mixtures of peat, perlite, and sand). In darkness at 15 and 20 °C high germination rates were obtained, both in vitro and in vivo (in vitro at 15 and 20 °C, max. 95%; in vivo at 15 °C, max. 73%; at 20 °C, max. 93%); however, at 15 °C germination was faster (after the first 20 days at 15 °C, max. 95%; at 20 °C, 73%). The .