tailieunhanh - The effect of drying and submersion pretreatment on adventitious shoot regeneration from hypocotyl explants of flax (Linum usitatissimum L.)

Hypocotyl explants of 3 flax cultivars were cultured for adventitious shoot regeneration in 3 different ways. Two pretreatment applications were compared with the routinely applied conventional regeneration protocol of culturing explants directly on Murashige and Skoog (MS) medium containing 1 mg L-1 6-benzylaminopurine (BAP) and mg L-1 naphthalene acetic acid (NAA). | Research Article Turk J Bot 34 (2010) 323-328 © TÜBİTAK doi: The effect of drying and submersion pretreatment on adventitious shoot regeneration from hypocotyl explants of flax (Linum usitatissimum L.) Mustafa YILDIZ*, Sebahattin ÖZCAN, Cansu TELCİ, Sibel DAY, Hande ÖZAT Ankara University, Faculty of Agriculture, Department of Field Crops, 06110 Dışkapı, Ankara - TURKEY Received: Accepted: Abstract: Hypocotyl explants of 3 flax cultivars were cultured for adventitious shoot regeneration in 3 different ways. Two pretreatment applications were compared with the routinely applied conventional regeneration protocol of culturing -1 explants directly on Murashige and Skoog (MS) medium containing 1 mg L 6-benzylaminopurine (BAP) and mg -1 L naphthalene acetic acid (NAA). In the first pretreatment application, explants kept in a sterile cabin under an air flow for 30 min were immersed in MS solution containing 1 mg L-1 BAP and mg L-1 NAA for 15 min; then pretreated explants were cultured on MS medium without any growth regulators (MS0). In the second pretreatment application, -1 explants were kept in a sterile cabin under air flow for 30 min and then immersed in MS solution containing 1 mg L -1 BAP and mg L NAA for 15 min. The pretreated explants were then transferred to a culture medium enriched with 1 mg L-1 BAP and mg L-1 NAA. Fresh and dry weights of hypocotyl explants, shoot regeneration percentage, shoot number per hypocotyl, shoot length, and total chlorophyll content were recorded. From the results, it could be seen that -1 treating explants before culture initiation for regeneration with a liquid MS medium containing 1 mg L BAP and -1 mg L NAA for 15 min after keeping hypocotyls under an air flow in a sterile cabin for 30 min gave rise to the highest scores for tissue culture response. Key words: In vitro shoot regeneration, Linum usitatissimum, pretreatment application Keten bitkisi (Linum .