tailieunhanh - Embryological and cytochemical features of Scilla autumnalis L.

Nucellar epidermal cells in the micropylar region of Scilla autumnalis L. differentiate into a nucellar cap. It secretes a PAS-positive exudate filling the micropyle. A group of cells at the chalazal end of the nucellus forms a hypostase. Close to the antipodals, the hypostase cells contain abundant starch grains, suggesting a function as a storage tissue. | Research Article Turk J Bot 34 (2010) 291-301 © TÜBİTAK doi: Embryological and cytochemical features of Scilla autumnalis L. Zeynep Mine COŞKUN1, Meral ÜNAL2 1İstanbul Bilim University, Health Services Vocational School, Department of Medical Laboratory Techniques Program, Esentepe, 34394, İstanbul - TURKEY 2Marmara University, Faculty of Arts & Sciences, Department of Biology, Göztepe, 34730, İstanbul - TURKEY Received: Accepted: Abstract: Nucellar epidermal cells in the micropylar region of Scilla autumnalis L. differentiate into a nucellar cap. It secretes a PAS-positive exudate filling the micropyle. A group of cells at the chalazal end of the nucellus forms a hypostase. Close to the antipodals, the hypostase cells contain abundant starch grains, suggesting a function as a storage tissue. Another ovular structure is the obturator. It secretes an exudate that stains deeply with PAS reaction, and it takes a role in directing the growth of pollen tubes toward the micropyle. Cytochemical tests in the mature embryo sac indicate the presence of high amounts of proteins, polysaccharides, and lipids in the cytoplasm of the egg, synergids, antipodals, and central cell, appearing to be the site of intensive synthetic activity. The presence of a PAS-positive wall completely surrounding the egg cell and synergids is an unusual feature worthy of mention. Antipodals undergo polytenisation to reach ploidy levels of up to 128n. Another embryological feature of S. autumnalis is the development of an Onagrad type of embryo and a helobial type of endosperm. Key words: Scilla autumnalis, embryo, endosperm, antipodals, cytochemistry Scilla autumnalis L. türünün embriyolojik ve sitokimyasal özellikleri Özet: S. autumnalis türünün mikropil tarafındaki nusellus epidermis hücreleri, nusellus şapkasını oluşturur. Nusellus epidermisi ve nusellus şapkası hücrelerinin hem çeperlerinin hem de sitoplazmalarının PAS pozitif reaksiyon verdiği .