tailieunhanh - In vitro propagation of endemic and endangered Muscari mirum from different explant types

A protocol for in vitro propagation of Muscari mirum Speta, an endangered geophyte of the family Hyacinthaceae, was developed. Bulb scale, scape, leaf, and immature embryo explants were cultured on different nutrient media compositions supplemented with various concentrations of plant growth regulators (BAP, NAA, TDZ, Picloram, 2,4-D). Scape explants produced callus only. | Research Article Turk J Bot 35 (2011) 37-43 © TÜBİTAK doi: In vitro propagation of endemic and endangered Muscari mirum from different explant types Ayşe Gül NASIRCILAR1, Semra MİRİCİ2,*, Özgül KARAGÜZEL3, Özkan EREN4, İbrahim BAKTIR5 1 Department of Biology Education, Faculty of Education, Akdeniz University, 07058 Antalya - TURKEY 2Department of Primary Education, Faculty of Education, Akdeniz University, 07058 Antalya - TURKEY 3West Mediterranean Agricultural Research Institute, Antalya - TURKEY 4Department of Biology, Faculty of Arts and Science, Adnan Menderes University, Aydın - TURKEY 5Department of Horticulture, Faculty of Agriculture, Akdeniz University, 07058 Antalya - TURKEY Received: Accepted: Abstract: A protocol for in vitro propagation of Muscari mirum Speta, an endangered geophyte of the family Hyacinthaceae, was developed. Bulb scale, scape, leaf, and immature embryo explants were cultured on different nutrient media compositions supplemented with various concentrations of plant growth regulators (BAP, NAA, TDZ, Picloram, 2,4-D). Scape explants produced callus only. Higher numbers of bulblets were obtained from bulb scale explants than embryo explants. The highest percentage of the bulb formation ( per explant) were obtained from bulb scale explants consisting of 4 scale segments cultured on Murashige and Skoog (MS) medium supplemented with 4 mg L–1 6–1 benzylaminopurine (BAP) and mg L α- naphthaleneacetic acid (NAA) after 5 months of culture initiation. Rooted bulblets which were more than 5 mm in diameter were transplanted into potting mixture of soil, vermiculite and perlite (1:1:1). The chromosome number of the root cells of in vitro induced bulblets was diploid 2n = 18. Key words: Muscari mirum, in vitro propagation, bulb scale, immature embryo Endemik ve tehlike altındaki Muscari mirum türünün farklı eksplant tiplerinden in vitro çoğaltımı Özet: Bu çalışmada Hyacinthaceae familyasına .