tailieunhanh - Comparative RAPD analysis and pollen structure studies of Bellis perennis L.

Random amplified polymorphic DNA (RAPD) is one of the easiest and most commonly used molecular techniques for genetic variability analysis. In the present study, the genetic diversity of analysis of Bellis perennis L. collected in 4 Turkish geographical locations was investigated by RAPD-PCR analysis. | Research Article Turk J Bot 34 (2010) 479-484 © TÜBİTAK doi: Comparative RAPD analysis and pollen structure studies of Bellis perennis L. Neslihan KAVALCIOĞLU1, Leyla AÇIK1, Münevver PINAR2 1 Gazi University, Science and Arts Faculty, Department of Biology, Ankara, 06500 - TURKEY 2Ankara University. Fen and Arts Faculty, Department of Biology, Ankara, 06500 - TURKEY Received: Accepted: Abstract: Random amplified polymorphic DNA (RAPD) is one of the easiest and most commonly used molecular techniques for genetic variability analysis. In the present study, the genetic diversity of analysis of Bellis perennis L. collected in 4 Turkish geographical locations was investigated by RAPD-PCR analysis. In addition to RAPD, pollen analysis of the 4 plant populations was also conducted. Out of 40 RAPD primers tested, 12 exhibited distinct banding patterns showing 45% to 79% polymorphism. Plants from these 4 localities were almost the same morphologically. Considerable differences were observed in the RAPD profile and pollen shape of plants from the Rize/Dereköy location. The results of this study clearly show that there are significant variations in the field collected populations of B. perennis from different geographic and climatic locations in Turkey. Environmental conditions may help us to determine the genomic structures of species. Key words: Bellis perennis, RAPD-PCR, pollen Bellis perennis L. türünün RAPD ve polen yapılarının karşılaştırılmalı analizi Özet: Genetik çeşitliliği ortaya çıkarmak için kullanılan Rastgele Çoğaltılmış Polimorfik DNA (RAPD) yöntemi çok yaygın kullanılan ve basit bir yöntemdir. Bu çalışmada Türkiye’nin dört farklı lokasyonundan toplanmış Bellis perennis L. türü RAPD-PZR yöntemi ile analiz edilmiştir. RAPD yöntemine ilave olarak dört populasyonun polen analizleri de yapılmıştır. Kullanılan 40 RAPD primerinden 12 primer polimorfik bulunmuş ve polimorfizm oranı % 45 ila % 79 arasında değişmiştir. .

• Nông nghiệp
• Bellis perennis
• Random amplified polymorphic DNA
• RAPD PCR analysis
• Genomic structures of species
• Rhaponticoides iconiensis