tailieunhanh - Removing lipid from crude collagen of pangasius hypophthalmus with supercritical carbon dioxide

The collagen product has high molecular weight and consists of and γ chain. The composition of collagen was: HP of mg/g, organic compounds of , moisture of , lipid of and ashes of . The characteristics satisfied the requirements of cosmetic, pharmaceutical and food applications. | REMOVING LIPID FROM CRUDE COLLAGEN OF PANGASIUS HYPOPHTHALMUS WITH SUPERCRITICAL CARBON DIOXIDE Le Thi Thu Huong1 , Nguyen Hoang Dzung2, Phan Dinh Tuan2. 1 Department of Biotechnological and Environmental Engineering, Lac Hong University 2 Department of Chemical Engineering, Ho Chi Minh City University of Technology, Vietnam, 268 Ly Thuong Kiet St., , Hochiminh City, Vietnam. Email:lethuhuong1976@ ABSTRACT Freeze-dried collagen from basa fish (Pangasius hypophthalmus) skin has been removed lipid with SC-CO2 equipment (Thar SFC ), the efficiency of the lipid extraction reached 90%, corresponding to the decrease of lipid content in collagen from to at conditions as follows: 200 bar, 42oC, 60 minutes, ethanol 10% and flow rate 10g/min. The lipid extraction yield increased when pressure, temperature, flow rate and ethanol content increased, respectively. The collagen product has high molecular weight and consists of 1, 2, and γ chain. The composition of collagen was: HP of mg/g, organic compounds of , moisture of , lipid of and ashes of . The characteristics satisfied the requirements of cosmetic, pharmaceutical and food applications. Keywords. collagen, supercritical carbon dioxide, pangasius hypophthalmus fish skin 1. INTRODUCTION Supercritical carbon dioxide (SC-CO2) fluid extraction has been applied in the commercial production of flavouring cosmetics, pharmaceuticals and food products. Examples are decaffeinated coffee, hop extract, extraction of turmeric essential oils, and ginger flavouring. In the oleo-industry, numerous researchers have tried extracting from seeds and refinement of plant oils with SC-CO2. There are several advantages in using SC-CO2 in industrial production. CO2 has several desirable properties, such as non- corrosion, non- toxicity, non-flammability and non explosibility. Because CO2 is stable chemically, it never reacts with other materials. Easy separation and removal