tailieunhanh - QTL mapping of some productivity and fibre traits in Gossypium arboreum

This study explains the construction of an intraspecific genetic linkage map of the A-genome diploid cotton with simple sequence repeat (SSR) and randomly amplified polymorphic DNA (RAPD) markers, using 180 F2 plants derived from the cross of 2 Gossypium arboreum cotton cultivars (Ravi × Entry-17). | Turkish Journal of Botany Turk J Bot (2013) 37: 802-810 © TÜBİTAK doi: Research Article QTL mapping of some productivity and fibre traits in Gossypium arboreum 1,2 1 1, Tayyaba SHAHEEN , Yusuf ZAFAR , Mehboob-ur RAHMAN * Plant Genomics and Molecular Breeding Laboratories, National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan 2 Department of Bioinformatics and Biotechnology, GC University, Faisalabad, Pakistan 1 Received: Accepted: Published Online: Printed: Abstract: This study explains the construction of an intraspecific genetic linkage map of the A-genome diploid cotton with simple sequence repeat (SSR) and randomly amplified polymorphic DNA (RAPD) markers, using 180 F2 plants derived from the cross of 2 Gossypium arboreum cotton cultivars (Ravi × Entry-17). Polymorphisms between the 2 parents were detected using 1089 pairs of SSR primers and 520 RAPD primers. In total, 34 pairs of SSR primer-amplified and 18 RAPD primer-amplified polymorphic loci were selected to survey the F2 population. In total, 52 polymorphic DNA fragments were generated and used to construct a linkage map with JoinMap . A total of 45 loci and 5 phenotypic traits were mapped at a logarithm of odds ratio of ≥ on 10 linkage groups. The total length of the map was 346 cM, and the average distance between adjacent markers was cM. Chromosome assignments were made according to previous studies. In total, 7 quantitative trait loci (QTLs), including 5 for productivity traits and 2 for fibre traits, were mapped. Identification of these QTLs will set the stage for initiating marker-assisted selection and map-based cloning in cotton. Key words: Diploid cotton, Gossypium arboreum, simple sequence repeats, genetic map, quantitative trait loci, fibre, productivity traits 1. Introduction A-genome species of cotton including Gossypium arboreum L. and .