tailieunhanh - Study of phylogenetic relationship of Turkish species of Matthiola (Brassicaceae) based on ISSR amplification

Matthiola is a taxonomically complex genus in which there are many problems, mostly with Matthiola longipetala and M. odoratissima. Matthiola species native to Turkey were collected from various locations in Anatolia, and their DNA was isolated. | Turkish Journal of Botany Turk J Bot (2016) 40: 130-136 © TÜBİTAK doi: Research Article Study of phylogenetic relationship of Turkish species of Matthiola (Brassicaceae) based on ISSR amplification 1, 2 3 4 5 6 Bekir DOĞAN *, Mustafa ÇELİK , Murat ÜNAL , Abdurrahman SEFALI , Esra MARTİN , Ayla KAYA 1 Department of Science Education, Faculty of Education, Necmettin Erbakan University, Konya, Turkey 2 Department of Biotechnology, Faculty of Science, Selçuk University, Konya, Turkey 3 Department of Biology Education, Faculty of Education, Yüzüncü Yıl University, Van, Turkey 4 Department of Science Education, Faculty of Education, Bayburt University, Bayburt, Turkey 5 Department of Biotechnology, Faculty of Science, Necmettin Erbakan University, Konya, Turkey 6 Department of Basic Pharmaceutical Sciences, Faculty of Pharmacy, Anadolu University, Eskişehir, Turkey Received: Accepted/Published Online: Final Version: Abstract: Matthiola is a taxonomically complex genus in which there are many problems, mostly with Matthiola longipetala and M. odoratissima. Matthiola species native to Turkey were collected from various locations in Anatolia, and their DNA was isolated. Revision studies performed on the basis of molecular data obtained from studies conducted in recent years have made the phylogenetic relationships and systematic positions of the taxa more apparent and reliable. Consequently, the remaining taxonomic problems among the species have been resolved through the use of DNA-based molecular analysis methods, which, unlike phenotype studies, are not affected by environmental factors. The inter-simple sequence repeat (ISSR) fingerprinting method was used in the study because its properties were considered to be more reliable and consistent than those of the randomly amplified polymorphic DNA method. DNA fragments were amplified through the use of ISSR .