tailieunhanh - Relationships between Sorghum bicolor (Poaceae) and its close relatives based on genomic in situ hybridization evidence

Sorghum bicolor (L.) Moench (sorghum) is a naturally well-established diploid species with 2n = 2x = 20. Here we provide evidence to identify the genomic relationships of S. bicolor and its extant relatives using genomic in situ hybridization. | Turkish Journal of Botany Turk J Bot (2017) 41: 11-24 © TÜBİTAK doi: Research Article Relationships between Sorghum bicolor (Poaceae) and its close relatives based on genomic in situ hybridization evidence 1, 1 2 Qing LIU *, Huan LIU , Jun WEN Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, . China 2 Department of Botany, National Museum of Natural History, Smithsonian Institution, Washington, DC, USA 1 Received: Accepted/Published Online: Final Version: Abstract: Sorghum bicolor (L.) Moench (sorghum) is a naturally well-established diploid species with 2n = 2x = 20. Here we provide evidence to identify the genomic relationships of S. bicolor and its extant relatives using genomic in situ hybridization. The genomic divergences between S. bicolor and its close relatives may be presented as: S. bicolor-S. × drummondii (Nees ex Steud.) Millsp. & Chase < S. bicolor-S. × almum Parodi < S. bicolor S. arundinaceum (Desv.) Stapf < S. bicolor-S. propinquum (Kunth) Hitchc. Sorghum arundinaceum probe signals showed spotted or painted patterns on the S. bicolor chromosomes, indicating that the degree of genomic divergence between S. bicolor and S. arundinaceum is distinct. It is reasonable to infer that S. bicolor might have diverged from S. arundinaceum in the early diversification history of the subgenus. The probe signal intensity of S. arundinaceum and S. propinquum is greater on the 17 chromosomes than on the remaining 23 chromosomes of S. halepense (L.) Pers., whereas S. bicolor and S. propinquum probes produced spotted or patchy signals on the 40 S. halepense chromosomes. Therefore, the degree of genomic divergence may be presented as: S. halepense-S. bicolor < S. halepense-S. arundinaceum < S. halepense-S. propinquum. Key words: Cereal crops, cultivated sorghum, genomic