tailieunhanh - Ameliorative role of β-estradiol against lead-induced oxidative stress and genotoxic damage in germinating wheat seedlings
In the present study, to determine the effects of β-estradiol on the ability of plants to tolerate lead toxicity, β-estradiol (10 µM) and lead ( mM), singly or in combination, were exogenously applied to wheat seeds. Although lead resulted in a marked increase in the activities of antioxidant enzymes, including superoxide dismutase, guaiacol peroxidase. | Turkish Journal of Botany Turk J Bot (2015) 39: 1051-1059 © TÜBİTAK doi: bot-1505-23 Research Article Ameliorative role of β-estradiol against lead-induced oxidative stress and genotoxic damage in germinating wheat seedlings 1, 2,3 2 4 2 5 Mucip GENİŞEL *, Hülya TÜRK , Serkan ERDAL , Yavuz DEMİR , Ebru GENÇ , İrfan TERZİ Department of Crop and Animal Production, Vocational High School, Ağrı İbrahim Çeçen University, Ağrı, Turkey 2 Department of Biology, Faculty of Science, Atatürk University, Erzurum, Turkey 3 East Anatolian High Technology Research and Application Center, Atatürk University, Erzurum, Turkey 4 Department of Biology, Kazım Karabekir Faculty of Education, Atatürk University, Erzurum, Turkey 5 Department of Biology, Faculty of Education, Dumlupınar University, Kütahya, Turkey 1 Received: Accepted/Published Online: Printed: Abstract: In the present study, to determine the effects of β-estradiol on the ability of plants to tolerate lead toxicity, β-estradiol (10 µM) and lead ( mM), singly or in combination, were exogenously applied to wheat seeds. Although lead resulted in a marked increase in the activities of antioxidant enzymes, including superoxide dismutase, guaiacol peroxidase, ascorbate peroxidase, and glutathione reductase (but not catalase), as well as an increase in the level of antioxidant compounds such as ascorbic acid and glutathione, this was insufficient to ameliorate the lead-induced oxidative injury or the superoxide anion, hydrogen peroxide, and malondialdehyde levels. However, β-estradiol was able to reduce the lead-induced oxidative damage and improved the antioxidant system. Similarly, β-estradiol reduced lead-induced α-amylase activity. The effects of lead toxicity on genetic material were also determined using the randomly amplified polymorphic DNA technique. While lead led to DNA damage in wheat seedlings, β-estradiol significantly
đang nạp các trang xem trước
