tailieunhanh - Machilus thunbergii extract inhibits inflammatory response in lipopolysaccharide-induced RAW264.7 murine macrophages via suppression of NF-κB and p38 MAPK activation

Machilus thunbergii Sieb. & Zucc. (Lauraceae) is a medicinal plant used to treat edema and pain in China and Taiwan. The anti-inflammatory effects of M. thunbergii were not clear. In this study, we evaluated the anti-inflammatory effects of M. thunbergii extract in LPS-stimulated RAW cells. | Turkish Journal of Biology Turk J Biol (2015) 39: 657-665 © TÜBİTAK doi: Research Article Machilus thunbergii extract inhibits inflammatory response in lipopolysaccharide-induced murine macrophages via suppression of NF-κB and p38 MAPK activation 1, 2, 2 2 3,4 4,5, Shu-Ju WU *, Wen-Bin LEN *, Chien-Yi HUANG , Chian-Jiun LIOU , Wen-Chung HUANG , Chwan-Fwu LIN ** Department of Nutrition and Health Sciences, Chang Gung University of Science and Technology, Kwei-Shan, Tao-Yuan, Taiwan, . 2 Department of Nursing, Chang Gung University of Science and Technology, Kwei-Shan, Tao-Yuan, Taiwan, . 3 Graduate Institute of Health Industry Technology, Chang Gung University of Science and Technology, Kwei-Shan, Tao-Yuan, Taiwan, . 4 Research Center for Industry of Human Ecology, Chang Gung University of Science and Technology, Kwei-Shan, Tao-Yuan, Taiwan, . 5 Department of Cosmetic Science, Chang Gung University of Science and Technology, Kwei-Shan, Tao-Yuan, Taiwan, 333, . 1 Received: Accepted/Published Online: Printed: Abstract: Machilus thunbergii Sieb. & Zucc. (Lauraceae) is a medicinal plant used to treat edema and pain in China and Taiwan. The anti-inflammatory effects of M. thunbergii were not clear. In this study, we evaluated the anti-inflammatory effects of M. thunbergii extract in LPS-stimulated RAW cells. The cells were pretreated with various doses ( µg/mL–100 µg/mL) of M. thunbergii extract and activated with LPS. We found that 25 µg/mL M. thunbergii extract inhibited nitric oxide (NO), prostaglandin E2 (PGE2), inducible nitric oxide synthetase (iNOS), and cyclooxygenase-2 (COX-2) expression, as well as levels of proinflammatory cytokines including interleukin (IL)-6 and tumor necrosis factor-alpha (TNF-α). Furthermore, M. thunbergii extract also suppressed translocation of nuclear transcription factor kappa-B (NF-κB)