tailieunhanh - Genetic integrity assessment of cryopreserved tomato (Lycopersicon esculentum Mill.) genotypes

Shoot tips of five tomato (Lycopersicon esculentum Mill.) genotypes were successfully cryopreserved by droplet vitrification. Recovered plants were studied for genetic stability by two different assays: amplified fragment length polymorphism (AFLP) using 4 primer combinations and sequencing of lycopene β-cyclase gene (LCY-B) from leaves. | Turkish Journal of Biology Research Article Turk J Biol (2015) 39: 638-648 © TÜBİTAK doi: Genetic integrity assessment of cryopreserved tomato (Lycopersicon esculentum Mill.) genotypes Ana COSTE*, Dana ŞUTEU, Ioan BĂCILĂ, Constantin DELIU, Sergiu VĂLIMĂREANU, Adela HALMAGYI Institute of Biological Research, branch of National Institute of Research and Development for Biological Sciences, Cluj-Napoca, Romania Received: Accepted/Published Online: Printed: Abstract: Shoot tips of five tomato (Lycopersicon esculentum Mill.) genotypes were successfully cryopreserved by droplet vitrification. Recovered plants were studied for genetic stability by two different assays: amplified fragment length polymorphism (AFLP) using 4 primer combinations and sequencing of lycopene β-cyclase gene (LCY-B) from leaves. The highest shoot regeneration after cryopreservation was 70% (Pontica) following 24 h osmoprotection in M sucrose and 30 min dehydration in plant vitrification solution 2 (PVS2). The data inferred from AFLP showed no genetic dissimilarities between in vitro regenerants derived from cryopreserved tissues compared with the noncryopreserved controls. Although a single nucleotide polymorphism, a G→T transversion, was identified in position 1139 in Capriciu and Darsirius, this was not due to the cryopreservation process itself, since it was detected in both cryopreserved and control samples. Thus, sequencing of LCY-B gene from leaves revealed no DNA damages after cryopreservation and subsequent in vitro regeneration. Our results indicate that cryostorage by droplet vitrification is an efficient and reliable technique to preserve the selected tomato genotypes and to regenerate true-to-type plants. Key words: AFLP, droplet-vitrification, genetic stability, LCY-B, tomato 1. Introduction Conservation of plant genetic resources is essential not just to maintain biodiversity, but .

• Sinh học
• Lycopersicon esculentum Mill
• Droplet vitrification
• Genetic stability
• Amplified fragment length polymorphism
• LCY B gene
• Tomato Lycopersicon esculentum Mill
• Plant growth
• Physiological parameters
• Fruit yield
• Semiarid regions
• Agri Fos 400
• Di potassium phosphonate
• Abnormal chromosomes
• Tomato meiocytes
• Glycerin gelatin liquid safranin mixture
• Triphenyl tetrazolium chloride
• Morphological structure
• K vitamin
• Calcium chloride
• Foliar application
• Fruit quality
• Borax influences plant growth
• Chuyển gen kháng sâu Cryiab
• Cây cà chua Lycopersicon esculentum Mill
• Sử dụng vi khuẩn Agrobacterium tumefaciens
• Vi khuẩn Agrobacterium tumefaciens
• Khả năng chuyển gen
• Naphthalene acetic acid
• Gibberellic acid on growth
• Fruit quality of tomato
• Tomato (Lycopersicon esculentum Mill
• Khảo sát khả năng sinh trưởng cà chua
• Phát triển của một số giống cà chua
• Giống cà chua Lycopersicon esculentum Mill
• Điều kiện nhà lưới
• Giống cà chua đen
• Plant growth promoting rhizobacteria
• FYM on the growth
• Yield attribute and yield
• Yield of tomato
• Genetic advance
• Genetic advance studies
• Quality traits
• Diverse genotypes of tomato
• Tomato (Lycopersicon esculentum Mill)
• Evaluation of growth of tomato
• Development of some varieties of tomato
• Nethouse conditions in Chau Doc
• Real field production
• New insecticides
• Aphis gossypii
• Bemecia tabaci
• Frankliniella schultzei
• Seed rates
• Damping off
• Population density
• Pots condition
• Neem leaves
• Tomato (Lycopersicon esculentum Mill )
• Growth and yield
• Biofertilizers on growth and yield
• Biofertilizers on growth
• Found for plant fresh weight
• Half life period
• Tomato fruits
• Household processing
• Reduction of residues of novaluron
• Calcium Metalosate
• Total dry matter production
• Fruit and yield
• Root knot nematodes
• Meloidogyne incognita
• Trichode rma isolates
• Tomato (Lycopersicon esculentum L Mill)
• Root knot nematode (Meloidogyne incognita)
• Nông nghiệp Việt Nam
• Tài liệu nông nghiệp
• Khả năng tích lũy chì
• Khả năng tích lũy cadimi
• Cây cà chua
• Alternaria solani
• Bio control agents
• Plant extract
• Fungicide and integrated disease management
• Plant Biology
• Lycopersicon esculentum
• Cold tolerance
• RNA interference
• Molecular biology
• Plant cells
• Journal of Botany
• Carbon nanofibers (CNFs)
• Gamma aminobutyric acid (GABA)
• Seed germination
• Salt stress
• báo cáo khao học
• báo cáo sinh học
• báo cáo về thủy sản
• các tài liệu về sinh học
• tài liệu nghiên cứu về vi sinh vật
• Biến nạp gen HbsAg
• Cà chua bi
• Agrobacterium tumefaciens
• Quy trình chuyển gen
• Hệ thống tái sinh
• kinh tế nông nghiệp
• chuyên đề nông học
• tài nguyên môi trường
• nuôi trồng thủy sản
• kinh tế xã hội
• nghiên cứu khoa học