tailieunhanh - Phenological variations of polyphenols in Smilax campestris (Smilacaceae)

Polyphenol profiles can suffer quali-quantitative modifications as the plant modifies its phenological condition. The objective of this work was to determine if there is a rhythm of production in the synthesis of polyphenols according to the phenological condition in the leaves, roots, and rhizomes of Smilax campestris Griseb. | Turkish Journal of Botany Research Article Turk J Bot (2013) 37: 350-354 © TÜBİTAK doi: Phenological variations of polyphenols in Smilax campestris (Smilacaceae) Ana Zulema RUGNA*, Alberto Angel GURNI, Marcelo Luis WAGNER Pharmacobotanics Chair, Pharmacology Department, Faculty of Pharmacy and Biochemistry, Buenos Aires University, Buenos Aires, Argentina Received: Accepted: Published Online: Printed: Abstract: Polyphenol profiles can suffer quali-quantitative modifications as the plant modifies its phenological condition. The objective of this work was to determine if there is a rhythm of production in the synthesis of polyphenols according to the phenological condition in the leaves, roots, and rhizomes of Smilax campestris Griseb. The plant material analysed corresponded to individuals of a colony of S. campestris collected in different phenological conditions. Standard methodology was used for the chromatographic profiles of flavonols (kaempferol, quercetin, isorhamnetin, and their glycosides) and proanthocyanidins (procyanidin and propelargonidin) and quantification of total phenol, condensed tannins, and flavonols. Appearance of metabolic changes was established in the studied organs of S. campestris according to the phenological condition. Key words: Smilax campestris, phenological condition, polyphenols, proanthocyanidins, flavonoids 1. Introduction Smilax campestris Griseb. (Smilacaceae) is a dioecious rhizomatous creeper with winter blooming; it is present from the north and east of South America to the Platense coast. In Argentina S. campestris is the most abundant and the most geographically distributed species of the gender; it is found in the northern provinces and it reaches the Plate Delta (Guaglianone & Gattuso, 1991; Mandrile & Bongiorno de Pfirter, 1991). It is a characteristic species from the forest, abundant in bushes and scrubland, .