tailieunhanh - Pathogenicity of Bursaphelenchus mucronatus in pine seedlings under greenhouse conditions

Bursaphelenchus mucronatus, a species closely related to the pinewood nematode (Bursaphelenchus xylophilus, a quarantine pest), was isolated from several wilted pine trees in different provinces of Turkey. | Turk J Agric For 31 (2007) 169-173 © TÜB‹TAK Pathogenicity of Bursaphelenchus mucronatus in Pine Seedlings under Greenhouse Conditions Süleyman AKBULUT1,*, Beflir YÜKSEL1, Metin SER‹N2, ‹smail BAYSAL1, Mahir ERDEM2 1 Düzce University, Faculty of Forestry, Konuralp Campus, 81620 Düzce - TURKEY 2 Republic of Turkey, Ministry of Environment and Forestry, Western Black Sea Forest Research Institute, Bolu - TURKEY Received: Abstract: Bursaphelenchus mucronatus, a species closely related to the pinewood nematode (Bursaphelenchus xylophilus, a quarantine pest), was isolated from several wilted pine trees in different provinces of Turkey. To understand the role of B. mucronatus in these wilting cases, the pathogenicity of B. mucronatus on 3-year-old seedlings of the 3 pine species most widespread in Turkey was investigated under greenhouse conditions. In all, 30 seedlings of each tree species were used for nematode inoculation. For inoculation, 1000-1100 nematodes in ml of distilled water were used per seedling. An additional 20 seedlings of each tree species were inoculated with an equal amount of distilled water and served as controls. The first complete wilting case was observed in the fifth week of the experiment. The highest rate of mortality occurred in Pinus sylvestris seedlings (83%), followed by P. nigra (47%). Only 2 seedlings of P. brutia wilted (7%). These results suggested that B. mucronatus was highly virulent to P. sylvestris seedlings. Key Words: Pinus sylvestris, Pinus nigra, Pinus brutia, Bursaphelenchus sp., pathogenicity, Turkey Bursaphelenchus mucronatus’un Sera Koflullar› Alt›nda Çam Fidanlar›ndaki Patojenli¤i Özet: Çam odun nematoduyla (Bursaphelenchus xylophilus, karantina listesindeki bir zararl›) benzerlikleri çok olan Bursaphelenchus mucronatus türü Türkiye’nin farkl› illerinde kurumufl olan baz› çam a¤açlar›ndan elde edilmifltir. B. mucronatus’un bu kurumalardaki rolünün belirlenmesi için en çok yay›l›fla sahip üç çam türünün .