tailieunhanh - Designing a bacterial biosensor for detection of mercury in water solutions

Due to increasing advances in physical and chemical techniques for the assessment of pollutants in the environment, there is an immediate demand for a bioassay that can report both the presence of an analyte and its biological effects. In accordance with this need, there has been a fast growth in whole-cell biosensor technology. | Turkish Journal of Biology Turk J Biol (2015) 39: 550-555 © TÜBİTAK doi: Research Article Designing a bacterial biosensor for detection of mercury in water solutions 1,2 3 4 5 Amir ROOINTAN , Nooshin SHABAB , Jamshid KARIMI , Alireza RAHMANI , 6 1,3, Mohammad Yousef ALIKHANI , Massoud SAIDIJAM * 1 Research Center for Molecular Medicine, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran 2 Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran 3 Department of Molecular Medicine and Genetics, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran 4 Department of Biochemistry and Nutrition, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran 5 Department of Environmental Health Engineering, Faculty of Health and Research Center for Health Sciences, Hamadan University of Medical Sciences, Hamadan, Iran 6 Department of Microbiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran Received: Accepted/Published Online: Printed: Abstract: Due to increasing advances in physical and chemical techniques for the assessment of pollutants in the environment, there is an immediate demand for a bioassay that can report both the presence of an analyte and its biological effects. In accordance with this need, there has been a fast growth in whole-cell biosensor technology. In this study we aimed to design a whole-cell bacterial biosensor to detect mercury in liquid solutions. The Pseudomonas pBS228 merR gene and its related promoter/operator was synthesized by Bioneer. The green fluorescence protein (GFP) gene was used as a reporter. GFP was cloned downstream of the merR gene. The construct, including the merR promoter, gene, and GFP, was cloned in a pUC19 vector and transferred into E. coli BL21 (DE3) .