tailieunhanh - Indirect organogenesis in summer squash (Cucurbita pepo L.)
Efficient plant regeneration via organogenesis was established for 2 summer squash (Cucurbita pepo L.) cultivars, viz. Bulum and Rumbo, using hypocotyl and cotyledon derived calli. | Turk J Agric For 31 (2007) 63-70 © TÜB‹TAK Indirect Organogenesis in Summer Squash (Cucurbita pepo L.) Shakti Prosad PAL1, Iftekhar ALAM1, M. ANISUZZAMAN, Kanak Kanti SARKER, Shamima Akhtar SHARMIN, Mohammad Firoz ALAM* Plant Biotechnology Laboratory, Department of Botany, Rajshahi University, Rajshahi-6205 - BANGLADESH Received: Abstract: Efficient plant regeneration via organogenesis was established for 2 summer squash (Cucurbita pepo L.) cultivars, viz. Bulum and Rumbo, using hypocotyl and cotyledon derived calli. Seeds were surface sterilized in HgCl2 for 5 min, and germinated in vitro in plant growth regulator free MS media. The maximum morphogenic callus induction rate (86%) was observed from a hypocotyl explant by culturing in MS medium supplemented with mg l-1 2,4-D. Calli size and fresh weight increased substantially through subculturing. The highest percentage of shoot regeneration (85%) and highest mean number of shoots () per culture were obtained with mg l-1 thidiazuron. Initiation of multiple shoots through organogenesis from the calli was histologically proven. Hypocotyl explants were more responsive than cotyledon explants in terms of callus induction and subsequent plant regeneration. Regenerated shoots were rooted in MS medium supplemented with mg l-1 IBA. About 70% of regenerated plantlets survived and showed new branch development under ex vitro conditions. Key Words: Cucurbita pepo, in vitro, organogenesis, summer squash, thidiazuron Abbreviations: 2,4-D: 2,4-Dichlorophenoxy acetic acid · BAP: 6-Benzylaminopurine · GA3: Gibberellic acid ·IBA: Indole-3-butyric acid · NAA: Naphthalene acetic acid · TDZ: Thidiazuron Introduction Cucurbita pepo L. includes a diverse range of vegetable crops including pumpkins, winter squash and various kinds of summer squash (Paris, 1989). In 2005, million US tons of squash production was noted worldwide (FAOSTAT, 2006). China contributes about 30% of the world’s .
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