tailieunhanh - Phytofabrication of biomolecule-coated metallic silver nanoparticles using leaf extracts of in vitro-raised bamboo species and its anticancer activity against human PC3 cell lines
The present study describes the biosynthesis of metallic silver nanoparticles (AgNPs) from silver ions using in vitro-grown leaf samples of Bambusa arundinacea (Ba) and Bambusa nutans (Bn) and their anticancer activity against human prostatic cancer cell lines (PC3). | Turkish Journal of Biology Turk J Biol (2015) 39: 223-232 © TÜBİTAK doi: Research Article Phytofabrication of biomolecule-coated metallic silver nanoparticles using leaf extracts of in vitro-raised bamboo species and its anticancer activity against human PC3 cell lines 1 1,2 3 1,2, Kalamegam KALAIARASI , Govindaraj PRASANNARAJ , Shivendra Vikram SAHI , Perumal VENKATACHALAM 1 Plant Genetic Engineering and Molecular Biology Lab, Department of Biotechnology, Periyar University, Periyar Palkalai Nagar, Salem, Tamil Nadu, India 2 Centre for Nanoscience and Nanotechnology, Periyar University, Salem, Tamil Nadu, India 3 Department of Biology, Western Kentucky University, Bowling Green, KY, USA Received: Accepted: Published Online: * Printed: Abstract: The present study describes the biosynthesis of metallic silver nanoparticles (AgNPs) from silver ions using in vitro-grown leaf samples of Bambusa arundinacea (Ba) and Bambusa nutans (Bn) and their anticancer activity against human prostatic cancer cell lines (PC3). The metallic AgNPs were synthesized at room temperature by treating the leaf extracts of Bambusa arundinacea and Bambusa nutans with 2 mM silver nitrate using the boiled method. The characterization of synthesized BaAgNPs and BnAgNPs was performed using UV-Vis spectrum (absorption peak at 365 nm), FTIR, TEM (spherical shape), SEM (size of 30–90 nm), and EDX analysis. Cell viability assays were carried out to determine the cytotoxicity effect of AgNPs on the PC3 cancer cell line and the Vero normal cell line. The IC50 values for BaAgNP- and BnAgNP-treated PC3 cancer cells were found to be µg/mL and µg/mL while the values for treated Vero cells were found to be µg/mL and µg/mL, respectively. The percentages of the apoptotic bodies by AO/EtBr staining were found to be 76% and 62% for BaAgNPs and BnAgNPs, respectively. The .
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