tailieunhanh - Antibacterial and cytotoxic properties of boron-containing dental composite

Secondary dental caries are one of the major reasons for restoration replacements. Incorporating antimicrobial properties into dental materials would limit the initiation and progression of dental caries. | Turkish Journal of Biology Turk J Biol (2015) 39: 417-426 © TÜBİTAK doi: Research Article Antibacterial and cytotoxic properties of boron-containing dental composite 1 2 1 1 Selami DEMİRCİ , Mustafa Sarp KAYA , Ayşegül DOĞAN , Şaban KALAY , 1 3 2 1, Nergis Özlem ALTIN , Ayşen YARAT , Serap Hatice AKYÜZ , Fikrettin ŞAHİN * 1 Department of Genetics and Bioengineering, Faculty of Engineering and Architecture, Yeditepe University, İstanbul, Turkey 2 Department of Pediatric Dentistry, Faculty of Dentistry, Marmara University, Nişantaşı, İstanbul, Turkey 3 Department of Basic Medical Sciences-Biochemistry, Faculty of Dentistry, Marmara University, Nişantaşı, İstanbul, Turkey Received: Accepted/Published Online: Printed: Abstract: Secondary dental caries are one of the major reasons for restoration replacements. Incorporating antimicrobial properties into dental materials would limit the initiation and progression of dental caries. In the current study, dental composites having 1%, 5%, and 10% (w/w) sodium pentaborate pentahydrate were prepared and analyzed for their mechanical properties, degree of monomer conversion (DC) rate, antibacterial effects against Streptococcus mutans, and biocompatibility with human dental pulp stem cells (hDPSCs). Incorporation of boron into the composites significantly decreased flexural strength and DC in a dose-dependent manner, but the value for 1% boron-containing composite still remained within acceptable levels. Compressive strength and diametral tensile strength were not found to be different from those of controls. Although no inhibition zone was detected in an agar-well diffusion assay for any materials tested, significant bacterial growth inhibition was obtained in a direct contact test for boron-containing composites. Immunocytochemical and lineage-specific gene expression analysis revealed that composites with boron content .