tailieunhanh - Estradiol receptors mediate estradiol-induced inhibition of mitochondrial Ca2+ efflux in rat caudate nucleus and brain stem

Our earlier studies found that in vitro estradiol modulates mitochondrial Ca2+ transport in discrete brain regions. The present study examined the role of estradiol receptors (ERs) in estradiol-induced inhibition of Ca2+ efflux from synaptosomal mitochondria isolated from rat caudate nuclei and brain stems. | Turkish Journal of Biology Research Article Turk J Biol (2015) 39: 328-334 © TÜBİTAK doi: Estradiol receptors mediate estradiol-induced inhibition of mitochondrial Ca2+ efflux in rat caudate nucleus and brain stem 1, 2 1 Snježana PETROVIĆ *, Maja MILOŠEVIĆ , Danijela RISTIĆ-MEDIĆ , 3 2 2 2 Nataša VELIČKOVIĆ , Dunja DRAKULIĆ , Ivana GRKOVIĆ , Anica HORVAT 1 Centre of Research Excellence in Nutrition and Metabolism, Institute for Medical Research, University of Belgrade, Belgrade, Serbia 2 Department of Molecular Biology and Endocrinology, Institute of Nuclear Sciences “Vinča”, University of Belgrade, Belgrade, Serbia 3 Institute for Biological Research “Siniša Stanković”, University of Belgrade, Belgrade, Serbia Received: Accepted: Published Online: Printed: Abstract: Our earlier studies found that in vitro estradiol modulates mitochondrial Ca2+ transport in discrete brain regions. The present study examined the role of estradiol receptors (ERs) in estradiol-induced inhibition of Ca2+ efflux from synaptosomal mitochondria isolated from rat caudate nuclei and brain stems. Radioactively labeled CaCl2 (– µCi 45CaCl2) was used for Ca2+ transport monitoring. The results revealed that in the presence of ER antagonist 7α,17β-[9[(4,4,5,5,5-pentafluoropentyl)sulfinyl]nonyl]estra1,3,5(10)-triene-3,17-diol (ICI 182,780) (1 µmol/L), the inhibitory effect of estradiol on mitochondrial Ca2+ efflux was more than 60% decreased, suggesting the involvement of ER in this mode of estradiol neuromodulatory action. When particular contributions of ERα and ERβ were tested, it was found that ERβ agonist 2,3-bis(4-hydroxy phenyl)-propionitrile (10 nmol/L) inhibited Ca2+ efflux more than 20%, while the inhibition with ERα agonist 4,4’,4’’-(4-propyl-[1H]-pyrazole-1,3,5-triyl)trisphenol (10 nmol/L) was about 10%, both compared to the control. Both agonists demonstrated .