tailieunhanh - Preliminary results on genome mapping of an Italia x Mercan grapevine population

This research was conducted to construct genetic linkage maps of Vitis (2n = 38) from grape populations (60 F1 + 2 parents) of crossing of Italia and Mercan (Vitis vinifera L.) cultivars. RAPD reactions were performed using a total of 300 RAPD primers. | Turk J Agric For 30 (2006) 273-280 © TÜB‹TAK Preliminary Results on Genome Mapping of an Italia x Mercan Grapevine Population Zeliha GÖKBAYRAK1, Cengiz ÖZER2, Gökhan SÖYLEMEZO⁄LU3,* 1 Çanakkale Onsekiz Mart University, Faculty of Agriculture, Department of Horticulture, 17020 Çanakkale - TURKEY 2 Tekirda¤ Viticultural Research Institute, Tekirda¤ - TURKEY 3 Ankara University Faculty of Agriculture Department of Horticulture, 06110 Ankara - TURKEY Received: Abstract: This research was conducted to construct genetic linkage maps of Vitis (2n = 38) from grape populations (60 F1 + 2 parents) of crossing of Italia and Mercan (Vitis vinifera L.) cultivars. RAPD reactions were performed using a total of 300 RAPD primers. The amplification products were scored from negatives as presence or absence. Only 59 loci for Italia and 55 loci for Mercan could be used for linkage analysis. Mapmaker/ was used for genetic linkage analysis with multipoint ordering among markers at LOD ≥ score. The map was developed by using the double pseudo-testcross mapping approach. Italia and Mercan resulted in 8 (maternal) and 6 (paternal) linkage groups, respectively. Loci placed on the linkage groups were further analyzed by regression and analyses of variances in order to determine possible linkages between the loci and morphological and disease resistance characteristics. According to the results, only 2 characters, flowering time and resistance to powdery mildew, were significantly linked (P ≥ ) to 1 and 2 marker loci, respectively. Other significant (P ≥ ) marker-QTL relationships were also found for various traits from regression analysis. Key Words: Grape, genetic map, linkage analysis, QTL, RAPD Italia x Mercan Asma Populasyonunda Genom Haritalamas› Üzerine ‹lk Sonuçlar Özet: Bu araflt›rmada, V. vinifera L. varyetesi olan Italia ve Mercan üzüm çeflitlerinin melezlenmesi ile elde edilen F1 populasyonu kullan›larak (60 F1 + 2 ebeveyn) genom haritas›n›n .

• Nông nghiệp
• Genetic map
• Linkage analysis
• Genetic linkage maps of Vitis
• Mercan Vitis vinifera L
• cultivars
• RAPD primers
• Linkage group
• Chickpea (Cicer arietinum L )
• QTLs for agronomic traits
• Genetic linkage map
• Map drawing
• Genomic map
• Conserved synteny
• Genome evolution
• Ensembl Compara databases
• BMC Plant Biology
• SLAF seq
• High density genetic map
• Seed weight
• Seed size
• Catalpa bungei
• RAD seq
• QTL mapping
• Leaf traits
• Plant growth
• Restriction site associated DNA sequencing
• Single nucleotide polymorphism
• Pyropia haitanensis
• Dense genetic linkage map
• Trait loci for economic traits
• Berry quality
• Next generation sequencing
• QTL analysis
• Quantitative trait loci
• Restriction site associated DNA
• Grain yield related traits
• Sesamum indicum L
• Specific locus amplified fragment sequencing
• High density
• Growth trait
• Genotyping by sequencing
• Win gene
• Homologous transformation sterility 1
• First flower node
• Candidate genesm
• Quantitative trait loc
• Plant Biology
• Integrated genetic map
• Sugar content
• Modern watermelon
• Horticultural qualities
• Structure variation
• Berry color
• Quantitative trait locus
• Candidate genes
• Vitis vinifera
• SNP based map
• Fruit quality related traits
• High density genetic mapping
• Rosaceae species
• High density genetic linkage map
• Muscat flavor
• Berry firmness
• Berry shape
• Grapevine breeding
• BMC Genomics
• Restoration of fertility
• Candidate gene
• Agronomic traits
• Elymus sibiricus
• Seed yield related traits
• Comparative genome analysis
• Brassica rapa
• Stalk color
• Barbarea vulgaris
• Crucifer tribe Cardamineae
• Upland cotton
• Fiber quality traits
• Quantitative trait loci mapping
• Single nucleotide polymorphism marker
• Stony hard
• Gene expression
• Crassostrea gigas
• Quantitative trait loci (QTL) mapping
• Quality traits
• Growth related traits
• Cyprinus carpio Haematopterus
• BMC Genetics
• Yellow lupin
• Linkage map
• Adaptation traits