tailieunhanh - Squalestatin-induced production of taxol and baccatin in cell suspension culture of yew (Taxus baccata L.)

Various elicitors have already been reported to enhance the production of taxol and related taxanes. In this study, effects of a fungal metabolite, squalestatin, on production of taxol and baccatin III were studied. Expressions of 4 key involved genes, ts, dbat, bapt, and dbtnbt, in suspension cultures of Taxus baccata were also detected using qPCR. | Turkish Journal of Biology Turk J Biol (2014) 38: 528-536 © TÜBİTAK doi: Research Article Squalestatin-induced production of taxol and baccatin in cell suspension culture of yew (Taxus baccata L.) 1 2,3, 2 2 2 Sayed-Asadollah AMINI , Leila SHABANI *, Ladan AFGHANI , Zohreh JALALPOUR , Majid SHARIFI-TEHRANI 1 Department of Biochemistry, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran 2 Department of Biology, Faculty of Basic Sciences, Shahrekord University, Shahrekord, Iran 3 Research Institute of Biotechnology, Shahrekord University, Shahrekord, Iran Received: Accepted: Published Online: Printed: Abstract: Various elicitors have already been reported to enhance the production of taxol and related taxanes. In this study, effects of a fungal metabolite, squalestatin, on production of taxol and baccatin III were studied. Expressions of 4 key involved genes, ts, dbat, bapt, and dbtnbt, in suspension cultures of Taxus baccata were also detected using qPCR. Results showed that application of squalestatin significantly increased taxol and baccatin III yields. Increased expressions of the genes were in accordance with measures of taxol and baccatin accumulations in cells and medium. Production of H2O2 has significant positive correlations with both gene expression and taxanes, indicating that the increase in H2O2 might be involved in the upregulation of the taxane production in yew under squalestatin treatment. Our results suggest that H2O2 is a key signaling component in the stimulation of taxane production in T. baccata cells induced by squalestatin. Key words: Baccatin, cell suspension, H2O2, squalestatin, taxol 1. Introduction Taxol (paclitaxel) is a potent antimitotic agent and an excellent active anticancer drug for the treatment of breast and ovarian cancers and AIDS-related Kaposi’s sarcoma (Sgadari et al., 2000). .