tailieunhanh - Styrian oil pumpkin pollen germinability at higher irradiation doses: optimization of the in vitro germination protocol and irradiation procedure

Protocols for haploid induction in cucurbits are based on pollination with irradiated pollen, but the induction frequency is low and the majority of obtained embryos are zygotic. The longevity of Cucurbita pepo L. pollen is short even under natural conditions; following irradiation, germinability is decreased even further. | Turkish Journal of Biology Research Article Turk J Biol (2014) 38: 516-522 © TÜBİTAK doi: Styrian oil pumpkin pollen germinability at higher irradiation doses: optimization of the in vitro germination protocol and irradiation procedure Kristina KOŠMRLJ, Damijana KASTELEC, Borut BOHANEC* Agronomy Department, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia Received: Accepted: Published Online: Printed: Abstract: Protocols for haploid induction in cucurbits are based on pollination with irradiated pollen, but the induction frequency is low and the majority of obtained embryos are zygotic. The longevity of Cucurbita pepo L. pollen is short even under natural conditions; following irradiation, germinability is decreased even further. This study was initiated to develop an optimal in vitro germination medium for styrian oil pumpkin (Cucurbita pepo L. subsp. pepo var. styriaca Greb.) pollen, which would enable accurate germination testing. Different pH values and the addition of sucrose, mannitol, and polyethylene glycol to the Brewbaker and Kwack germination medium were tested. The optimum medium condition was pH 9 and (w/v) sucrose, while other tested components were not efficient. Using the optimized medium, X-ray–irradiated (100–700 Gy) pollen germinability was assessed under 2 air humidity conditions. Germinability of pollen irradiated at room humidity (RH) was generally lower than that of pollen irradiated at high humidity (HH). A major variability in pollen size (diameters ranged from to µm) and 2 subgroups were found in the pollen population. Following irradiation, HH conditions allowed germination of larger pollen grains than those of the nonirradiated control and RH. Key words: Cucurbita pepo subsp. pepo var. styriaca, media composition, X-ray irradiation, pollen diameter, haploid induction 1. Introduction An .

• Sinh học
• Cucurbita pepo subsp
• pepo var
• styriaca
• Media composition
• X ray irradiation
• Pollen diameter
• Haploid induction
• BMC Plant Biology
• Twin embryo
• In vivo haploid induction
• Haploid induction rate
• Twin embryo kernel rate
• Flow cytometry
• Doubled haploid
• Crop improvement
• Plant species
• Reliable method
• Cesium 137
• Cobalt 60
• Embryo rescue
• Haploid plant
• X ray
• Plant Biology
• Double haploid inducer
• Paternal infiltration
• Chromosome elimination
• Brassica napus
• Embryo culture
• Ovule culture
• Ploidy level
• SSR marker
• In vitro ovule culture
• Chromosome counting
• Haploid plants
• Anther culture
• Water chestnut
• Ascorbic acid
• Trapa sp
• Genus Capsicum covers several dozen species
• Embryo induction
• Incubation length
• Homozygous doubled haploid
• Capsicum genotypes
• Journal of Botany
• Pollen embryo
• Chromosome doubling
• Flue cured tobacco Genetically homozygous lines
• Haploid aseptic seedlings